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Table 2 Primers for qPCR assay

From: Effects of urea plus nitrate pretreated rice straw and corn oil supplementation on fiber digestibility, nitrogen balance, rumen fermentation, microbiota and methane emissions in goats

Microbial Species

Primer Sets (5′→3′)

Product size, bp

References

Protozoa

F: GCTTTCGWTGGTAGTGTATT;

R: CTTGCCCTCYAATCGTWCT

223

[47]

Fungi

F:GAGGAAGTAAAAGTCGTAACAAGGTTTC;

R:CAAATTCACAAAGGGTAGGATGATT

121

[48]

Bacteria

F: CGGCAACGAGCGCAACCC;

R: CCATTGTAGCACGTGTGTAGCC

146

[48]

Methanogens

F: GGATTAGATACCCSGGTAGT;

R: GTTGARTCCAATTAAACCGCA

192

[49]

Selected groups of bacteria

 Fibrobacter succinogenes

F:GTTCGGAATTACTGGGCGTAAA;

R: CGCCTGCCCCTGAACTATC

121

[48]

 Ruminococcus albus

F:CCCTAAAAGCAGTCTTAGTTCG;

R: CCTCCTTGCGGTTAGAACA

176

[50]

 Ruminococcus flavefaciens

F: GAACGGAGATAATTTGAGTTTACTTAGG;

R: CGGTCTCTGTATGTTATGAGGTATTACC

132

[48]

 Selenomonas ruminantium

F: CAATAAGCATTCCGCCTGGG;

R: TTCACTCAATGTCAAGCCCTGG

138

[51]