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Fig. 6 | Journal of Animal Science and Biotechnology

Fig. 6

From: Exacerbated conceptus signaling does not favor establishment of pregnancy in beef cattle

Fig. 6

Effect of iP4 supplementation on ISG15 mRNA expression in PBMCs of beef cows. Animals detected in estrus (D0) were assigned randomly to receive a single injection of vehicle (Non-iP4) or 150 mg of long acting progesterone (iP4) on D4 and transfer of 5 in vitro produced blastocysts on D7. Conceptus signaling in cows diagnosed as pregnant at ultrasound exam on D30 (iP4; n = 10 and Non-iP4; n = 15) was monitored by abundance of ISG15 in peripheral blood mononuclear cells (PBMCs) isolated on D14, D16, D18 and D20. Data were analyzed by split-plot ANOVA and only the significant effect was reported. Data are represented as Box-plot using least squares means ± SEM over the time. The average abundance of ISG15 (between D14 and D20) was greater in iP4 supplemented cows than those Non-iP4 supplemented (mean is represented by the cross in the graphic)

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