Fig. 6
From: Exacerbated conceptus signaling does not favor establishment of pregnancy in beef cattle
Effect of iP4 supplementation on ISG15 mRNA expression in PBMCs of beef cows. Animals detected in estrus (D0) were assigned randomly to receive a single injection of vehicle (Non-iP4) or 150 mg of long acting progesterone (iP4) on D4 and transfer of 5 in vitro produced blastocysts on D7. Conceptus signaling in cows diagnosed as pregnant at ultrasound exam on D30 (iP4; n = 10 and Non-iP4; n = 15) was monitored by abundance of ISG15 in peripheral blood mononuclear cells (PBMCs) isolated on D14, D16, D18 and D20. Data were analyzed by split-plot ANOVA and only the significant effect was reported. Data are represented as Box-plot using least squares means ± SEM over the time. The average abundance of ISG15 (between D14 and D20) was greater in iP4 supplemented cows than those Non-iP4 supplemented (mean is represented by the cross in the graphic)