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Table 3 Plasma biomarkers and free amino acids at d 2, 7, 21, 42, and 50 of age in calves born to cows offered a control diet (CC and CM, n = 7/ group) or CON supplemented with ethyl-cellulose rumen-protected Met (MC and MM, n = 7/ group; Mepron® at 0.09% of diet DM; Evonik Nutrition & Care GmbH, Germany) during the last 28 d of pregnancy

From: Maternal supply of methionine during late-pregnancy enhances rate of Holstein calf development in utero and postnatal growth to a greater extent than colostrum source

Item

Maternal diet

Colostrum type

 

P value1

CON

MET

CON

MET

SEM

M

C

T

M × C

M × T

C × T

Biomarker, mmol/L

 Glucose

6.83

6.89

6.83

6.88

0.43

0.82

0.84

< 0.01

0.53

0.01

0.70

 Cholesterol

1.86

1.97

1.90

1.94

0.12

0.30

0.69

< 0.01

0.43

0.38

0.55

 NEFA

0.17

0.19

0.17

0.19

0.05

0.48

0.43

< 0.01

0.29

0.52

0.10

 Urea

4.60

4.84

4.60

4.84

0.42

0.20

0.22

< 0.01

0.53

0.29

0.87

 BHBA

0.11

0.13

0.11

0.12

0.04

0.06

0.21

< 0.01

0.60

0.93

0.41

 Creatinine

92.6

93.8

93.3

93.1

3.14

0.68

0.91

< 0.01

0.38

0.80

0.11

Amino acids, μmol/L

 His

5.37

6.14

5.89

5.98

0.34

0.38

0.84

< 0.01

0.51

0.74

0.99

 Ile

10.6

11.5

10.7

11.4

0.69

0.34

0.52

< 0.01

0.86

0.32

0.95

 Leu

15.3

16.9

15.7

16.4

1.0

0.29

0.63

< 0.01

0.64

0.38

0.94

 Lys

10.2

10.5

10.1

10.7

0.64

0.73

0.48

< 0.01

0.58

0.86

0.89

 Met

1.95

2.10

1.85b

2.20a

0.13

0.37

0.04

< 0.01

0.03

0.44

0.43

 Phe

3.99

4.02

3.92

4.09

0.19

0.91

0.56

< 0.01

0.01

0.09

0.70

 Thr

11.8

11.9

12.0

11.6

0.83

0.94

0.72

< 0.01

0.95

0.76

0.97

 Val

23.6

26.0

24.1

25.5

1.14

0.15

0.40

< 0.01

0.70

0.57

0.85

 Ala

21.6

22.2

21.5

22.2

1.1

0.65

0.62

< 0.01

0.09

0.35

0.79

 Arg

13.6

14.2

13.2

14.7

0.68

0.50

0.13

< 0.01

0.87

0.02

0.99

 Asn

4.03

4.54

4.18

4.38

0.31

0.25

0.65

< 0.01

0.76

0.79

0.89

 Asp

1.03

1.07

1.05

1.05

0.05

0.52

0.90

< 0.01

0.14

0.71

0.85

 Glu

6.58b

7.73a

7.11

7.16

0.39

0.03

0.92

< 0.01

0.95

0.03

0.04

 Gln

27.5b

31.2a

29.0

29.6

0.98

0.01

0.62

< 0.01

0.42

0.23

0.83

 Gly

27.8

27.1

26.5

28.4

1.1

0.67

0.19

< 0.01

0.25

0.35

0.99

 Pro

9.95

10.3

9.88

10.4

0.47

0.50

0.38

< 0.01

0.69

0.74

0.88

 Ser

8.35

8.23

7.84

8.74

0.35

0.82

0.08

< 0.01

0.36

0.31

0.96

 Tau

0.95

0.92

0.89

0.98

0.04

0.58

0.08

< 0.01

0.73

0.01

0.08

 Tyr

4.28

4.68

4.34

4.62

0.26

0.28

0.45

0.01

0.51

0.33

0.78

 Orn

0.71

0.76

0.69

0.78

0.04

0.37

0.12

< 0.01

0.43

0.35

0.78

 3-methyl-His

0.06

0.05

0.06

0.05

0.01

0.52

0.86

< 0.01

0.06

0.95

0.14

  1. 1M, maternal diet effect, C colostrum type effect, T time effect.
  2. a,bMeans on the same row differ (P ≤ 0.05).