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Table 3 Plasma biomarkers and free amino acids at d 2, 7, 21, 42, and 50 of age in calves born to cows offered a control diet (CC and CM, n = 7/ group) or CON supplemented with ethyl-cellulose rumen-protected Met (MC and MM, n = 7/ group; Mepron® at 0.09% of diet DM; Evonik Nutrition & Care GmbH, Germany) during the last 28 d of pregnancy

From: Maternal supply of methionine during late-pregnancy enhances rate of Holstein calf development in utero and postnatal growth to a greater extent than colostrum source

Item Maternal diet Colostrum type   P value1
CON MET CON MET SEM M C T M × C M × T C × T
Biomarker, mmol/L
 Glucose 6.83 6.89 6.83 6.88 0.43 0.82 0.84 < 0.01 0.53 0.01 0.70
 Cholesterol 1.86 1.97 1.90 1.94 0.12 0.30 0.69 < 0.01 0.43 0.38 0.55
 NEFA 0.17 0.19 0.17 0.19 0.05 0.48 0.43 < 0.01 0.29 0.52 0.10
 Urea 4.60 4.84 4.60 4.84 0.42 0.20 0.22 < 0.01 0.53 0.29 0.87
 BHBA 0.11 0.13 0.11 0.12 0.04 0.06 0.21 < 0.01 0.60 0.93 0.41
 Creatinine 92.6 93.8 93.3 93.1 3.14 0.68 0.91 < 0.01 0.38 0.80 0.11
Amino acids, μmol/L
 His 5.37 6.14 5.89 5.98 0.34 0.38 0.84 < 0.01 0.51 0.74 0.99
 Ile 10.6 11.5 10.7 11.4 0.69 0.34 0.52 < 0.01 0.86 0.32 0.95
 Leu 15.3 16.9 15.7 16.4 1.0 0.29 0.63 < 0.01 0.64 0.38 0.94
 Lys 10.2 10.5 10.1 10.7 0.64 0.73 0.48 < 0.01 0.58 0.86 0.89
 Met 1.95 2.10 1.85b 2.20a 0.13 0.37 0.04 < 0.01 0.03 0.44 0.43
 Phe 3.99 4.02 3.92 4.09 0.19 0.91 0.56 < 0.01 0.01 0.09 0.70
 Thr 11.8 11.9 12.0 11.6 0.83 0.94 0.72 < 0.01 0.95 0.76 0.97
 Val 23.6 26.0 24.1 25.5 1.14 0.15 0.40 < 0.01 0.70 0.57 0.85
 Ala 21.6 22.2 21.5 22.2 1.1 0.65 0.62 < 0.01 0.09 0.35 0.79
 Arg 13.6 14.2 13.2 14.7 0.68 0.50 0.13 < 0.01 0.87 0.02 0.99
 Asn 4.03 4.54 4.18 4.38 0.31 0.25 0.65 < 0.01 0.76 0.79 0.89
 Asp 1.03 1.07 1.05 1.05 0.05 0.52 0.90 < 0.01 0.14 0.71 0.85
 Glu 6.58b 7.73a 7.11 7.16 0.39 0.03 0.92 < 0.01 0.95 0.03 0.04
 Gln 27.5b 31.2a 29.0 29.6 0.98 0.01 0.62 < 0.01 0.42 0.23 0.83
 Gly 27.8 27.1 26.5 28.4 1.1 0.67 0.19 < 0.01 0.25 0.35 0.99
 Pro 9.95 10.3 9.88 10.4 0.47 0.50 0.38 < 0.01 0.69 0.74 0.88
 Ser 8.35 8.23 7.84 8.74 0.35 0.82 0.08 < 0.01 0.36 0.31 0.96
 Tau 0.95 0.92 0.89 0.98 0.04 0.58 0.08 < 0.01 0.73 0.01 0.08
 Tyr 4.28 4.68 4.34 4.62 0.26 0.28 0.45 0.01 0.51 0.33 0.78
 Orn 0.71 0.76 0.69 0.78 0.04 0.37 0.12 < 0.01 0.43 0.35 0.78
 3-methyl-His 0.06 0.05 0.06 0.05 0.01 0.52 0.86 < 0.01 0.06 0.95 0.14
  1. 1M, maternal diet effect, C colostrum type effect, T time effect.
  2. a,bMeans on the same row differ (P ≤ 0.05).
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