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Table 1 Developmental parameters and blood biomarkers at birth in calves born to cows offered a control diet (CON, n = 39) or CON supplemented with ethyl-cellulose rumen-protected Met (MET, n = 42; Mepron® at 0.09% of diet DM; Evonik Nutrition & Care GmbH, Germany) during the last 28 d of pregnancy

From: Maternal supply of methionine during late-pregnancy enhances rate of Holstein calf development in utero and postnatal growth to a greater extent than colostrum source

Item Maternal diet Sex P value1
CON MET Bull Heifer SEM M S M × S
Body weight, kg 42.1b 44.1a 44.9a 41.3b 0.70 0.04 < 0.01
Hip height, cm 79.6b 81.3a 81.0 79.8 0.53 0.02 0.11
Hip width, cm 16.3b 16.5ab 16.7a 16.1b 0.24 0.74 0.05 0.03
Wither height, cm 75.9b 77.8a 77.1 76.6 0.47 < 0.01 0.38
Body length, cm 110 112 112a 109b 1.0 0.30 0.04
Blood biomarkers, mmol/L
 Glucose 4.54 4.85 4.82 4.57 0.68 0.72 0.78
 Cholesterol 0.59 0.67 0.64 0.62 0.03 0.09 0.69
 NEFA 0.94 1.20 1.03 1.11 0.10 0.07 0.55
 Urea 5.63 6.31 6.47 5.47 0.39 0.19 0.06
 BHBA 0.07 0.05 0.06 0.06 0.01 0.11 0.48
 Creatinine 206 225 197 234 17.0 0.42 0.11
Amino acids, μmol/L
 His 8.81a 6.51b 7.59 7.73 0.61 0.01 0.86
 Ile 3.95 3.92 4.78a 3.08b 0.51 0.97 0.02
 Leu 7.51 6.74 8.43a 5.82b 0.80 0.47 0.02
 Lys 3.49a 2.54b 3.37 2.66 0.35 0.05 0.14
 Met 2.41 1.80 2.21 2.01 0.24 0.06 0.54
 Phe 4.63 4.20 4.40 4.44 0.32 0.30 0.93
 Thr 4.72 3.69 4.66 3.75 0.66 0.24 0.30
 Val 14.22 13.11 15.60a 11.74b 1.11 0.45 0.01
 Ala 69.71 50.40 70.81a 49.30b 7.79 0.07 0.05
 Arg 6.79 6.29 7.01 6.07 0.55 0.49 0.21
 Asn 2.10a 1.60b 2.08 1.62 0.18 0.04 0.06
 Asp 1.00 1.06 1.09 0.96 0.11 0.63 0.35
 Glu 3.04 3.37 3.68a 2.73b 0.26 0.33 0.01
 Gln 43.35 37.60 45.24 36.03 3.95 0.22 0.06
 Gly 46.18 44.84 47.75 43.27 3.66 0.78 0.36
 Pro 10.87 10.96 12.14a 9.69b 0.92 0.94 0.05
 Ser 7.30 7.52 8.57a 6.25b 0.80 0.84 0.04
 Tau 2.51 2.39 2.65 2.25 0.37 0.81 0.42
 Tyr 3.40 2.78 2.96 3.22 0.25 0.08 0.44
 Orn 0.26 0.22 0.27 0.21 0.02 0.18 0.06
 3-methyl-His 0.35 0.31 0.32 0.33 0.04 0.48 0.79
  1. 1M, maternal diet effect; S, bull or heifer effect
  2. a,bMeans on the same row differ (P ≤ 0.05).