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Table 1 Developmental parameters and blood biomarkers at birth in calves born to cows offered a control diet (CON, n = 39) or CON supplemented with ethyl-cellulose rumen-protected Met (MET, n = 42; Mepron® at 0.09% of diet DM; Evonik Nutrition & Care GmbH, Germany) during the last 28 d of pregnancy
| Item | Maternal diet | Sex | P value1 | |||||
|---|---|---|---|---|---|---|---|---|
| CON | MET | Bull | Heifer | SEM | M | S | M × S | |
| Body weight, kg | 42.1b | 44.1a | 44.9a | 41.3b | 0.70 | 0.04 | < 0.01 | – |
| Hip height, cm | 79.6b | 81.3a | 81.0 | 79.8 | 0.53 | 0.02 | 0.11 | – |
| Hip width, cm | 16.3b | 16.5ab | 16.7a | 16.1b | 0.24 | 0.74 | 0.05 | 0.03 |
| Wither height, cm | 75.9b | 77.8a | 77.1 | 76.6 | 0.47 | < 0.01 | 0.38 | – |
| Body length, cm | 110 | 112 | 112a | 109b | 1.0 | 0.30 | 0.04 | – |
| Blood biomarkers, mmol/L | ||||||||
| Glucose | 4.54 | 4.85 | 4.82 | 4.57 | 0.68 | 0.72 | 0.78 | – |
| Cholesterol | 0.59 | 0.67 | 0.64 | 0.62 | 0.03 | 0.09 | 0.69 | – |
| NEFA | 0.94 | 1.20 | 1.03 | 1.11 | 0.10 | 0.07 | 0.55 | – |
| Urea | 5.63 | 6.31 | 6.47 | 5.47 | 0.39 | 0.19 | 0.06 | – |
| BHBA | 0.07 | 0.05 | 0.06 | 0.06 | 0.01 | 0.11 | 0.48 | – |
| Creatinine | 206 | 225 | 197 | 234 | 17.0 | 0.42 | 0.11 | – |
| Amino acids, μmol/L | ||||||||
| His | 8.81a | 6.51b | 7.59 | 7.73 | 0.61 | 0.01 | 0.86 | – |
| Ile | 3.95 | 3.92 | 4.78a | 3.08b | 0.51 | 0.97 | 0.02 | – |
| Leu | 7.51 | 6.74 | 8.43a | 5.82b | 0.80 | 0.47 | 0.02 | – |
| Lys | 3.49a | 2.54b | 3.37 | 2.66 | 0.35 | 0.05 | 0.14 | – |
| Met | 2.41 | 1.80 | 2.21 | 2.01 | 0.24 | 0.06 | 0.54 | – |
| Phe | 4.63 | 4.20 | 4.40 | 4.44 | 0.32 | 0.30 | 0.93 | – |
| Thr | 4.72 | 3.69 | 4.66 | 3.75 | 0.66 | 0.24 | 0.30 | – |
| Val | 14.22 | 13.11 | 15.60a | 11.74b | 1.11 | 0.45 | 0.01 | – |
| Ala | 69.71 | 50.40 | 70.81a | 49.30b | 7.79 | 0.07 | 0.05 | – |
| Arg | 6.79 | 6.29 | 7.01 | 6.07 | 0.55 | 0.49 | 0.21 | – |
| Asn | 2.10a | 1.60b | 2.08 | 1.62 | 0.18 | 0.04 | 0.06 | – |
| Asp | 1.00 | 1.06 | 1.09 | 0.96 | 0.11 | 0.63 | 0.35 | – |
| Glu | 3.04 | 3.37 | 3.68a | 2.73b | 0.26 | 0.33 | 0.01 | – |
| Gln | 43.35 | 37.60 | 45.24 | 36.03 | 3.95 | 0.22 | 0.06 | – |
| Gly | 46.18 | 44.84 | 47.75 | 43.27 | 3.66 | 0.78 | 0.36 | – |
| Pro | 10.87 | 10.96 | 12.14a | 9.69b | 0.92 | 0.94 | 0.05 | – |
| Ser | 7.30 | 7.52 | 8.57a | 6.25b | 0.80 | 0.84 | 0.04 | – |
| Tau | 2.51 | 2.39 | 2.65 | 2.25 | 0.37 | 0.81 | 0.42 | – |
| Tyr | 3.40 | 2.78 | 2.96 | 3.22 | 0.25 | 0.08 | 0.44 | – |
| Orn | 0.26 | 0.22 | 0.27 | 0.21 | 0.02 | 0.18 | 0.06 | – |
| 3-methyl-His | 0.35 | 0.31 | 0.32 | 0.33 | 0.04 | 0.48 | 0.79 | – |
