Table 3 Primer sequences used for quantitative RT-PCR analyses
From: Neonatal vitamin A injection promotes cattle muscle growth and increases oxidative muscle fibers
Gene name | Accession no. | Product size, bp | Direction | Sequence (5′→3′) |
|---|---|---|---|---|
PAX3 | NM_001206818.1 | 200 | Forward | AGTGAGTTCCATCAGCCGCATC |
Reverse | TCTTCAGGGGCAAGTCTGGTTC | |||
PAX7 | XM_015462509.1 | 107 | Forward | CGGGCATGTTTAGCTGGGAGA |
Reverse | TCTGAGCACTCGGCTAATCGAAC | |||
MYOD | NM_001040478.2 | 111 | Forward | GAACTGCTACGACCGCACTTACT |
Reverse | GAGATGCGCTCCACGATGCT | |||
MYF5 | XM_015470879.1 | 93 | Forward | CCCACCAGCCCCACCTCAAGT |
Reverse | GTAGACGCTGTCAAAACTGCTGCT | |||
MYOG | NM_001111325.1 | 131 | Forward | CTCAACCAGGAGGAGCGCGAC |
Reverse | TTGGGGCCAAACTCCAGTGCG | |||
18S | NR_036642.1 | 118 | Forward | CCTGCGGCTTAATTTGACTC |
Reverse | AACTAAGAACGGCCATGCAC | |||
PGC1A | NM_177945.3 | 200 | Forward | TGATTAGTTGAGCCCTTGCCG |
Reverse | TGCCAGGAGTTTGGTTGTGAT |