Fig. 1

In vitro adipocyte differentiation. Adipocytes were obtained from the subcutaneous adipose tissue of three five-day-old Chinese Erhualian pigs and collected at four differentiation stages: D0, D2, D4, and D8. (a) Enlarged adipocyte photos during the differentiation (D0, D2, D4, and D8; D8 with Oil Red O staining) (200×). (b) Triglyceride contents at the different stages of differentiation were measured using a triglycerol assay kit. (c) Absorbance values of destained Oil Red O extracted from the cells at 510 nm. Data are represented as mean ± SD, n = 3 per group. * P < 0.05; ** P < 0.01; *** P < 0.001