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Table 3 Relative abundance (%) of 10 targeted rumen bacteria species and the 16S rRNA gene copy numbers of the total rumen bacterial community in the rumen solids-fraction of the most- (n = 6) and the least- (n = 6) efficient beef cattle on RFI testing for 70 d during the finishing period

From: Residual feed intake in beef cattle and its association with carcass traits, ruminal solid-fraction bacteria, and epithelium gene expression

Items1

RFI2

SE3

Sex

SE4

P-value

Most-efficient animals

Least-efficient animals

Steers

Heifers

RFI

Sex

RFI × sex

Target bacterial species

 A. lipolytica

1.80 × 10−4

9.00 × 10−5

0.27

1.20 × 10−4

1.30 × 10− 4

0.31

0.48

0.96

0.45

 B. proteoclasticus

1.91 × 10−2

1.12 × 10− 2

0.22

1.33 × 10− 2

1.60 × 10− 2

0.25

0.47

0.80

0.66

 E. ruminantium

9.13 × 10-2a

4.18 × 10-2b

0.10

9.09 × 10-2a

4.20 × 10-2b

0.11

0.05

0.05

0.84

 F. succinogenes

2.90 × 10-4a

1.00 × 10-4b

0.13

3.00 × 10-4a

0.90 × 10-4b

0.14

0.03

0.02

0.79

 M. elsdenii

7.00 × 10-5a

3.00 × 10-5b

0.12

4.00 × 10−5

5.00 × 10−5

0.12

0.04

0.73

0.35

 P. bryantii

4.31 × 10−2

3.52 × 10−2

0.14

5.37 × 10−2

2.82 × 10− 2

0.16

0.67

0.20

0.40

 S. ruminantium

1.25 × 10−1

1.17 × 10−1

0.08

1.21 × 10−1

1.20 × 10−1

0.09

0.80

0.97

0.95

 S. amylolytica

0.70 × 10-4b

3.80 × 10-4a

0.14

2.40 × 10−4

1.20 × 10−4

0.14

< 0.01

0.16

0.83

 S. bovis

5.63 × 10−3

6.75 × 10−3

0.25

6.30 × 10− 3

6.03 × 10− 3

0.29

0.83

0.96

0.48

 S. dextrinosolvens

4.81 × 10−2

1.22 × 10−2

0.48

1.38 × 10− 2

4.26 × 10− 2

0.56

0.41

0.49

0.28

Bacteria density

 The 16S rRNA gene copy numbers (log10)/ng DNA5

8.03b

8.07a

0.01

8.05

8.05

0.01

0.05

0.89

0.60

  1. Means with different superscripts (a, b) within row represent differences among overall RFI or sex effects (P ≤ 0.05)
  2. 1Data were logit transformed to ensure normality of residuals
  3. 2RFI residual feed intake
  4. 3SE standard error of the mean for RFI effect
  5. 4SE standard error of the mean for sex effect
  6. 516S rRNA gene copy number (log10)/ng DNA