Residual feed intake phenotype and gender affect the expression of key genes of the lipogenesis pathway in subcutaneous adipose tissue of beef cattle

Table 3 Effect of RFI on the expression of lipogenic genes in adipose tissue from cattle divergent for RFI^b (n = 9 bulls, n = 8 heifers)

Gene^b,c	Bulls			Heifers			P-value
Gene^b,c	HRFI^a	LRFI	SD^e	HRFI	LRFI	SD	RFI	G^d	RFI × G
ACAT1	2.55	3.88	0.68	3.52	4.38	0.3	0.12	0.0002	0.65
ACACA	2.39	2.21	0.48	4.98	6.63	0.31	0.16	< 0.001	0.12
ACLY	5.15	4.34	0.29	3.48	3.24	0.54	0.38	0.23	0.32
ELOVL6	2.74	2.05	0.43	3.93	3.41	0.41	0.45	0.01	0.62
FASN	4.35	4.23	0.35	4.21	5.9	0.39	0.38	0.02	0.23
SLC2A4	0.96	1.42	0.33	3.85	5.21	0.52	0.01	< 0.0001	0.34
HMGCOR	2.46	1.83	0.39	5.4	6.77	0.81	0.39	0.0002	0.13
HMGCS2	1.31	2.1	0.28	6.21	4.9	0.58	0.2	0.01	0.03
SCD	4.64	3.65	0.35	6.81	7.6	0.61	0.98	0.003	0.69
SREBPC1	2.07	2.45	0.41	4.82	4.55	0.66	0.4	0.0008	0.54

^aHigh RFI is feed inefficient and low RFI is feed efficient
^bGene expression values were calculated by normalizing cycle threshold (Ct) values for target genes to Ct values of the reference genes after adjustment for efficiencies and interplate variation and converted to values relative to the mean Ct value within each data set using GENEX (MultiD Analyses, AB, Gothenberg, Sweden)
^cSee Table 1 for gene names
^dG Gender
^eSD Standard Deviation

ISSN: 2049-1891