Table 1 Sequences of bovine oligonucleotide primers used for real-time reverse transcription PCR
Gene symbol | Accession numbera | Primer sequence (5′ →3′)b |
|---|---|---|
SLC2A4 d | NM_174604.1 | F: GGGACTGGTACCCATGTACG |
R: AGGAGGAGTGGCCATAAGGT | ||
ACAT1 d | NM_001046075.1 | F: TCAACGGAGGAGCTGTTTCT |
R: TGCAAATACTGGCAAGACCA | ||
HMGCoAS d | NM_001045883.1 | F: AGAACGTCTGCCCTCTTTCA |
F: TACAAGGCTGCTGTGTCCAG | ||
SREBP1c d | NM_001113302.1 | F: CTACATCCGCTTCCTTCAGC |
R: TTCAGCGATTTGCTTTTGTG | ||
FASN d | NM_001012669.1 | F: TCATCCCCCTGATGAAGAAG |
R: AACTCCACAGGTGGGAACAG | ||
ACLY d | NM_001037457.1 | F: CAAGAAGGCAGACCAGAAGG |
R: CTGGGCGGTACAGCTTAGAG | ||
ELOVL6 d | NM_001102155.1 | F: GGAAAGCAACGAAAGCTGAC |
R: TGGGTTGTGTGTTTGCTCAT | ||
SCD d | NM_173959.4 | F: CGACGTGGCTTTTTCTTCTC |
R: GATACCATGGCACGAGTGTG | ||
ACACA d | NM_174224.2 | F: TGTCCGAAACGTCGATTTTTG |
R: ACGACCTGGTTGCTGTGATAGA | ||
HMGCoAR d | NM_001105613.1 | F: TGAGGGAGAACATTGCTCGT |
R: ACATGATTTCGAGCTGACGC | ||
RFLPO c | NM_001012682 | F: CAACCCTGAAGTGCTTGACAT |
R: AGGCAGATGGATCAGCCA | ||
UTX2 c | NM_001037471 | F: TGTGGCCCTTGGATATGGTT |
R: GGTTGTCGCTGAGCTCTGTG | ||
TPB c | NM_001075742 | F: CCTAAAGACCATTGCACTTCG |
R: CTTCACTCTTGGCTCCTGTG | ||
TOP2B c | XM_001254709 | F: CCGATGATGATGACGACAAT |
R: TGCTATGGGAGATGCTTTGA | ||
B-actin c | U39357 | F: CTCACGGAGCGTGGCTACA |
R: GCCATCTCCTGCTCGAAGTC |