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Fig. 2 | Journal of Animal Science and Biotechnology

Fig. 2

From: N-carbamylglutamate restores nitric oxide synthesis and attenuates high altitude-induced pulmonary hypertension in Holstein heifers ascended to high altitude

Fig. 2

Expression of endotheline-1 and endothelial nitric oxide synthases mRNA and protein in lung tissues. Control = healthy Holstein heifers, HAPH = Holstein heifers with high altitude pulmonary hypertension. Means ± SEM (n = 3), *P < 0.05; **P < 0.01. Relative mRNA levels of endotheline-1 (ET-1) and endothelial nitric oxide synthases (eNOS) in lung tissues. The expression of β-actin served as an internal control. Bar graph representing the relative ET-1 and eNOS protein levels. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) protein expression served as an internal control. Western blot showing ET-1 and eNOS protein levels in lung tissues

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Journal of Animal Science and Biotechnology

ISSN: 2049-1891