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Table 2 Primer pairs used to scan EDN3 gene for polymorphism

From: Molecular cloning and characterization of the endothelin 3 gene in black bone sheep

Primer No. Primer sequences (5′→3′) Binding regions Product size, bp Ta, °C Location (UCSC)
1 F-ATTAGGTGAACGCTGACA
R-CCCTCCAACTGCAGATGC
5 Flanking region, partial exon 1 579 56 61,296,366–61,296,944
2 F-CGCTCTGAAGTTTGTGACG
R-AGACCCTTACACCCACCATC
Partial exon1, partial intron1 782 56 61,295,675–61,296,460
3 F-ACGCTGTGGAGTAAGTGAGA
R-TAGAGACCAGGAAAGCAAAT
Partial intron1, exon 2, partial intron2 1,335 52 61,294,558–61,296,896
4 F-AGCCACCTTGTTTTACCG
R-GCCCAAGTTCCCTAAAGC
Intron 2 1,268 52 61,293,086–61,294,353
5 F-TGGTAGGGTGGCAAGATA
R-TCCAGGTCAAATGTAGGC
Intron 2 914 50 61,285,341–61,286,254
6 F-GTAGGAAGGCATCTTATTGG
R-GAGTAACGCAGGTGAACG
Partial intron2, exon 3, partial intron3 1,231 50 61,275,666–61,276,905
7 F-CTCAGCCTTCGGAAACTAT
R-CTCCTCTGCGTTTTTATTGT
Partial intron3, exon4, partial intron 4 946 50 61,274,254–61,275,198
8 F-CCAGACAAAGCAAGTAGG
R-GAATGCGTTTCCGAGATA
Partial intron4, exon5 1,407 51 61,271,825–61,273,231
  1. Note: UCSC genome browser on Feb.2010, ISGC Ovis_aries _1.0/oviAri. http://genome.ucsc.edu/cgi-bin/hgBlat