Table 2 Primer pairs used to scan EDN3 gene for polymorphism
From: Molecular cloning and characterization of the endothelin 3 gene in black bone sheep
Primer No. | Primer sequences (5′→3′) | Binding regions | Product size, bp | Ta, °C | Location (UCSC) |
|---|---|---|---|---|---|
1 | F-ATTAGGTGAACGCTGACA R-CCCTCCAACTGCAGATGC | 5 Flanking region, partial exon 1 | 579 | 56 | 61,296,366–61,296,944 |
2 | F-CGCTCTGAAGTTTGTGACG R-AGACCCTTACACCCACCATC | Partial exon1, partial intron1 | 782 | 56 | 61,295,675–61,296,460 |
3 | F-ACGCTGTGGAGTAAGTGAGA R-TAGAGACCAGGAAAGCAAAT | Partial intron1, exon 2, partial intron2 | 1,335 | 52 | 61,294,558–61,296,896 |
4 | F-AGCCACCTTGTTTTACCG R-GCCCAAGTTCCCTAAAGC | Intron 2 | 1,268 | 52 | 61,293,086–61,294,353 |
5 | F-TGGTAGGGTGGCAAGATA R-TCCAGGTCAAATGTAGGC | Intron 2 | 914 | 50 | 61,285,341–61,286,254 |
6 | F-GTAGGAAGGCATCTTATTGG R-GAGTAACGCAGGTGAACG | Partial intron2, exon 3, partial intron3 | 1,231 | 50 | 61,275,666–61,276,905 |
7 | F-CTCAGCCTTCGGAAACTAT R-CTCCTCTGCGTTTTTATTGT | Partial intron3, exon4, partial intron 4 | 946 | 50 | 61,274,254–61,275,198 |
8 | F-CCAGACAAAGCAAGTAGG R-GAATGCGTTTCCGAGATA | Partial intron4, exon5 | 1,407 | 51 | 61,271,825–61,273,231 |