Table 1 Primers used for 5′ and 3′ RACE
From: Molecular cloning and characterization of the endothelin 3 gene in black bone sheep
Primer name | Sequences (5′→3′) | Ta, °C | PCR |
|---|---|---|---|
5´ Gene specific primer | ACCGTCTCCTTGGTGTCCCCCTCCGAT | 65 | Normal - kit protocol |
5´ Gene specific nested primer | ATCCTGCGGCGGAGGTCACAGCGAG | 75 | Touchdown |
3´ Gene specific primer | GCTGAGGTGTTAGCCTTGACCAAATGC | 65 | Normal - kit protocol |
3´ Gene specific nested primer | TGGAAAGGACTGATGTGCCAGCGAGAT | 75 | Touchdown |