Effects of dietary phosphorous supplementation on laying performance, egg quality, bone health and immune responses of laying hens challenged with Escherichia coli lipopolysaccharide

Table 2 Primers used for relative real-time PCR^a

Gene	Primer sequence (5′ → 3′) ^b	GenBank accession no.
GAPDH	F: GGTGGAGGAATGGCTGTCA	NM_204305
GAPDH	R: CCTAGGATACACAGAGGACCAGGTT	NM_204305
IL-1β	F: ACTGGGCATCAAGGGCTA	HM179638
IL-1β	R: GGTAGA AGATGA AGCGGGTC	HM179638
IL-6	F: TTTATGGAGAAGACCGTGAGG	HM179640
IL-6	R: TGTGGCAGATTGGTAACAGAG	HM179640
IL-10	F: GCTGTCACCGCTTCTTCACCT	EF554720.1
IL-10	R: GGCTCACTTCCTCCTCCTCATC	EF554720.1

GAPDH Glyceraldehyde-3-phosphate dehydrogenase, IL-1β Interleukin-1 beta, IL-6 Interleukin-6, IL-10 Interleukin 10
^aPrimers designed by Primer Express software (Applied Biosystems, Foster City, CA)
^bF Forward, R reverse

ISSN: 2049-1891