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Table 1 Composition of the experimental laying hen diet

From: Effects of dietary phosphorous supplementation on laying performance, egg quality, bone health and immune responses of laying hens challenged with Escherichia coli lipopolysaccharide

Items Dietary treatments
  LPS – – + +
  NPP,% 0.12 0.40 0.12 0.40
Ingredient, %
 Corn   61.29 60.45 61.29 60.45
 Soybean meal   27.21 27.29 27.21 27.29
 Soybean oil   0.97 1.21 0.97 1.21
 Limestone   9.73 8.59 9.73 8.59
 Dicalcium phosphate   0 1.65 0 1.65
 Salt   0.30 0.30 0.30 0.30
 Trace mineral premixa   0.20 0.20 0.20 0.20
 Vitamin premixb   0.02 0.02 0.02 0.02
 DL-Methionine   0.16 0.17 0.16 0.17
 Choline chloride   0.12 0.12 0.12 0.12
 Total   100 100 100 100
Nutrient composition, %
 ME, Mcal/kg   2.7 2.7 2.7 2.7
 CP   17 17 17 17
 Calcium   3.5 3.5 3.5 3.5
 Nonphytate phosphorus (NPP)   0.12 0.40 0.12 0.40
 Lysine   0.86 0.86 0.86 0.86
 Methionine+Cystine   0.65 0.65 0.65 0.65
 Tryptophan   0.21 0.21 0.21 0.21
 Threonine   0.66 0.66 0.66 0.66
  1. aMineral premix provided per kilogram of diet: Mn, 100 mg; Fe, 80 mg; Zn, 75 mg; Cu, 8 mg; I, 0.35 mg; Se, 0.15 mg
  2. bVitamin premix provided per kilogram of diet: vitamin A, 12,500 IU; vitamin D3, 2,500 IU; vitamin E, 30 IU; vitamin K3, 2.65 mg; vitamin B1, 2 mg; vitamin B2, 6 mg; vitamin B12, 0.025 mg; biotin, 0.0325 mg; folic acid, 1.25 mg; pantothenic acid, 12 mg; niacin, 50 mg
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