Effect of CD14/TLR4 antagonist on GnRH/LH secretion in ewe during central inflammation induced by intracerebroventricular administration of LPS

Table 1 Specific primers used in real-time PCR for determining the expression of housekeeping genes and genes of interests

Gene	GenBank Acc. No.	Amplicon Size, bp	Sequence (5′ → 3′)
Gene	GenBank Acc. No.	Amplicon Size, bp	Forward	Reverse
GAPDH ^a	NM_001034034	134	AGAAGGCTGGGGCTCACT	GGCATTGCTGACAATCTTGA
ACTB ^b	U39357	168	CTTCCTTCCTGGGCATGG	GGGCAGTGATCTCTTTCTGC
PPIC ^c	NM_001076910	131	ACGGCCAAGGTCTTCTTTG	TATCCTTTCTCTCCCGTTGC
GnRH ^d	U02517	123	GCCCTGGAGGAAAGAGAAAT	GAGGAGAATGGGACTGGTGA
GnRH-R ^e	NM_001009397	150	TCTTTGCTGGACCACAGTTAT	GGCAGCTGAAGGTGAAAAAG
TLR4 ^fg	AY957615	117	GGTTCCCAGAACTGCAAGTG	GGATAGGGTTTCCCGTCAGT
IL-1β ^h	X54796.1	137	CAGCCGTGCAGTCAGTAAAA	GAAGCTCATGCAGAACACCA

ISSN: 2049-1891