Ileum terminal antibiotic infusion affects jejunal and colonic specific microbial population and immune status in growing pigs

Table 3 Primer pairs for host genes

Gene^a	Forward primer, 5′→3’	Reverse primer, 5′→3’	Reference	Annealing temp, °C
β-actin	AGAGCGCAAGTACTCCGTGT	ACATCTGCTGGAAGGTGGAC	[70]	60
TLR2	TCACTTGTCTAACTTATCATCCTCTTG	TCAGCGAAGGTGTCATTATTGC	[71]	60
TLR4	TCAGTTCTCACCTTCCTCCTG	GTTCATTCCTCACCCAGTCTTC	[72]	60
TLR5	CAGCGACCAAAACAGATTGA	TGCTCACCAGACAGACAACC	[71]	60
IL-8	ACTGGCTGT TGCCTTCTT	CAGTT CTCTTCAAAAATATCTG	[73]	60
IL-10	GTCCGACTCAACGAAGAAGG	GCCAGGAAGATCAGGCAATA	[70]	60
IFN-γ	TCCAGCGCAAAGCCATCAGTG	ATGCTCTCTGGCCTTGGAACATAGT	[74]	60
TNF-α	CCACGCTCTTCTGCCTACTGC	GCTGTCCCTCGGCTTTGAC	[73]	60
MUC1	GGTACCCGGCTGGGGCATTG	GGTAGGCATCCCGGGTCGGA	[70]	60
MUC2	CTGCTCCGGGTCCTGTGGGA	CCCGCTGGCTGGTGCGATAC	[70]	60
OCLN	ATGCTTTCTCAGCCAGCG TA	AAG GTTCCATAGCCTCGGTC	[73]	60
ZO-1	GAGGATGGTCACACCGTGGT	GGAGGATGCTGTTGTCTCGG	[73]	60

^aTLR2 toll-like receptor2, TLR4 toll-like receptor 4, TLR5 toll-like receptor 5, IL-8 interleukin-8, IL-10 interleukin-10, IFN-γ interferon-γ, TNF-α tumor necrosis factor-α, ZO-1 zonula occludens-1, MUC1 mucin-1; MUC2 mucin-2; OCLN occludin

ISSN: 2049-1891