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Table 4 Effect of β-mannanase on pig serum glucose, insulin, acute phase protein, and cytokine concentrationsa,b

From: Lipopolysaccharide immune stimulation but not β-mannanase supplementation affects maintenance energy requirements in young weaned pigs

Treatment Control dietc Enzyme dietd Contrast P-value
Estimate SEM Estimate SEM
Glucose, mmol/L 7.70 0.46 7.41 0.25 0.585
Insulin, pmol/L 88.92 10.99 83.35 6.06 0.664
Insulin:Glucose 11.56 1.21 11.08 0.67 0.734
Acute phase protein, mg/mL
 Haptoglobin 1.57 0.22 1.02 0.14 0.050
 MBLe 125.4 8.6 117.5 5.2 0.445
Cytokine, pg/mLf
 GM-CSF 17.01 13.01 14.56 7.85 0.874
 IL-1α 35.01 6.81 7.50 4.27 0.004
 IL-1β 1064 598 1138 361 0.917
 IL-1ra 428.6 257.5 673.7 161.5 0.435
 IL-2 328.4 157.6 228.8 95.0 0.596
 IL-4 995.5 545.6 711.6 329.0 0.662
 IL-6 190.2 61.5 98.72 38.59 0.230
 IL-8 263.1 95.4 402.2 57.5 0.230
 IL-10 499.4 159.1 332.9 95.9 0.383
 IL-12 1570 173 1730 104 0.439
 IL-18 1994 546 1380 329 0.350
 TNFα 24.29 39.90 52.30 25.02 0.563
  1. aBlood was collected on d 8 of the experiment 6 h post-feeding, prior to the immune challenge beginning on d 10
  2. bn = 10 pigs per treatment
  3. cControl diet was a corn, soybean mean, and soy hulls based diet containing 1.33% β-mannans, and did not contain β-mannanase enzyme. Pigs on the control (CON) treatment were fed the control diet and estimates are representative of the CON treatment only
  4. dEnzyme diet was the control diet supplemented with 0.056% β-mannanase (Hemicell™ HT-D, Elanco Animal Health, Guelph, ON, Canada; endo-1,4-β-mannanase (160 × 106 units/kg) from Paenibacillus alvei). Pigs on the enzyme (ENZ) and immune system stimulation (ISS) treatments were fed the enzyme diet. Estimates are representative of the ENZ and ISS treatments prior to immune stimulation
  5. eMannose binding lectin A (MBL)
  6. fGranulocyte-macrophage colony-stimulating factor (GM-CSF); interleukin-1α (IL-1α); interleukin-1β (IL-1β); interleukin-1 receptor antagonist (IL-1ra); interleukin-2 (IL-2); interleukin-4 (IL-4); interleukin-6 (IL-6); interleukin-8 (IL-8); interleukin-10 (IL-10); interleukin-12 (IL-12); interleukin-18 (IL-18); tumor necrosis factor alpha (TNFα)