Lipopolysaccharide immune stimulation but not β-mannanase supplementation affects maintenance energy requirements in young weaned pigs

Table 4 Effect of β-mannanase on pig serum glucose, insulin, acute phase protein, and cytokine concentrations^a,b

Treatment	Control diet^c		Enzyme diet^d		Contrast P-value
Treatment	Estimate	SEM	Estimate	SEM	Contrast P-value
Glucose, mmol/L	7.70	0.46	7.41	0.25	0.585
Insulin, pmol/L	88.92	10.99	83.35	6.06	0.664
Insulin:Glucose	11.56	1.21	11.08	0.67	0.734
Acute phase protein, mg/mL
Haptoglobin	1.57	0.22	1.02	0.14	0.050
MBL^e	125.4	8.6	117.5	5.2	0.445
Cytokine, pg/mL^f
GM-CSF	17.01	13.01	14.56	7.85	0.874
IL-1α	35.01	6.81	7.50	4.27	0.004
IL-1β	1064	598	1138	361	0.917
IL-1ra	428.6	257.5	673.7	161.5	0.435
IL-2	328.4	157.6	228.8	95.0	0.596
IL-4	995.5	545.6	711.6	329.0	0.662
IL-6	190.2	61.5	98.72	38.59	0.230
IL-8	263.1	95.4	402.2	57.5	0.230
IL-10	499.4	159.1	332.9	95.9	0.383
IL-12	1570	173	1730	104	0.439
IL-18	1994	546	1380	329	0.350
TNFα	24.29	39.90	52.30	25.02	0.563

^aBlood was collected on d 8 of the experiment 6 h post-feeding, prior to the immune challenge beginning on d 10
^bn = 10 pigs per treatment
^cControl diet was a corn, soybean mean, and soy hulls based diet containing 1.33% β-mannans, and did not contain β-mannanase enzyme. Pigs on the control (CON) treatment were fed the control diet and estimates are representative of the CON treatment only
^dEnzyme diet was the control diet supplemented with 0.056% β-mannanase (Hemicell™ HT-D, Elanco Animal Health, Guelph, ON, Canada; endo-1,4-β-mannanase (160 × 10⁶ units/kg) from Paenibacillus alvei). Pigs on the enzyme (ENZ) and immune system stimulation (ISS) treatments were fed the enzyme diet. Estimates are representative of the ENZ and ISS treatments prior to immune stimulation
^eMannose binding lectin A (MBL)
^fGranulocyte-macrophage colony-stimulating factor (GM-CSF); interleukin-1α (IL-1α); interleukin-1β (IL-1β); interleukin-1 receptor antagonist (IL-1ra); interleukin-2 (IL-2); interleukin-4 (IL-4); interleukin-6 (IL-6); interleukin-8 (IL-8); interleukin-10 (IL-10); interleukin-12 (IL-12); interleukin-18 (IL-18); tumor necrosis factor alpha (TNFα)

ISSN: 2049-1891