Lipopolysaccharide immune stimulation but not β-mannanase supplementation affects maintenance energy requirements in young weaned pigs

Table 2 Experimental diet ingredient and analyzed nutrient composition (as-fed basis)

Item	Control diet	Enzyme diet
Ingredient, % of diet
Corn	47.33	47.27
Soybean meal, (dehulled, solvent extracted)	38.40	38.40
Soybean hulls	10.00	10.00
Soybean oil	1.85	1.85
Limestone	1.04	1.04
Monocalcium phosphate	0.60	0.60
Vitamin premix^a	0.33	0.33
Trace mineral premix^b	0.20	0.20
Salt	0.25	0.25
Hemicell HT-D^c	0.00	0.06
Calculated composition, % of diet
SID Lys	1.18	1.18
SID Met	0.32	0.32
SID Thr	0.75	0.75
SID Trp	0.26	0.26
SID Cys + Met	0.62	0.62
β-mannan^e	1.33	1.33
Analyzed composition, % of diet
DM	86.75	87.17
GE, Mcal/kg	4.03	4.00
CP	22.28	21.83
EE^d	4.02	3.97
Starch	29.34	30.89
NDF	12.17	11.83
ADF	6.97	6.79
endo-1,4-β-mannanase^f, IU/kg	Below detectable limit ^g	150,000

^aProvided per kilogram of complete diet: 6,614 IU of vitamin A; 827 IU of vitamin D; 26 IU of vitamin E; 2.6 mg of vitamin K; 29.8 mg of niacin; 16.5 mg of pantothenic acid; 5.0 mg of riboflavin; 0.023 mg of vitamin B₁₂
^bProvided per kilogram of complete diet: Zn, 165 mg as ZnSO₄; Fe, 165 mg as FeSO₄; Mn, 39 mg as MnSO₄; Cu, 17 mg as CuSO₄; I, 0.3 mg as Ca (IO₃)₂; and Se, 0.3 mg as Na₂SeO₃
^cHemicell™ HT-D, Elanco Animal Health, Guelph, ON, Canada; endo-1,4-β-mannanase (160 × 10⁶ units/kg) from Paenibacillus alvei
^dAcid hydrolyzed ether extract
^eβ-mannan concentration was calculated using values reported in Shastak et al. [70]
^fendo-1,4-β-mannanase activity. 1 IU = the amount of enzyme which generates 0.72 micrograms of reducing sugars per minute from a mannose-containing substrate at pH 7.0 and temperature of 40 °C
^gThe lowest detectable limit was 15,000 IU/kg

ISSN: 2049-1891