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Table 2 Experimental diet ingredient and analyzed nutrient composition (as-fed basis)

From: Lipopolysaccharide immune stimulation but not β-mannanase supplementation affects maintenance energy requirements in young weaned pigs

Item Control diet Enzyme diet
Ingredient, % of diet
 Corn 47.33 47.27
 Soybean meal, (dehulled, solvent extracted) 38.40 38.40
 Soybean hulls 10.00 10.00
 Soybean oil 1.85 1.85
 Limestone 1.04 1.04
 Monocalcium phosphate 0.60 0.60
 Vitamin premixa 0.33 0.33
 Trace mineral premixb 0.20 0.20
 Salt 0.25 0.25
 Hemicell HT-Dc 0.00 0.06
Calculated composition, % of diet
 SID Lys 1.18 1.18
 SID Met 0.32 0.32
 SID Thr 0.75 0.75
 SID Trp 0.26 0.26
 SID Cys + Met 0.62 0.62
 β-mannane 1.33 1.33
Analyzed composition, % of diet
 DM 86.75 87.17
 GE, Mcal/kg 4.03 4.00
 CP 22.28 21.83
 EEd 4.02 3.97
 Starch 29.34 30.89
 NDF 12.17 11.83
 ADF 6.97 6.79
 endo-1,4-β-mannanasef, IU/kg Below detectable limit g 150,000
  1. aProvided per kilogram of complete diet: 6,614 IU of vitamin A; 827 IU of vitamin D; 26 IU of vitamin E; 2.6 mg of vitamin K; 29.8 mg of niacin; 16.5 mg of pantothenic acid; 5.0 mg of riboflavin; 0.023 mg of vitamin B12
  2. bProvided per kilogram of complete diet: Zn, 165 mg as ZnSO4; Fe, 165 mg as FeSO4; Mn, 39 mg as MnSO4; Cu, 17 mg as CuSO4; I, 0.3 mg as Ca (IO3)2; and Se, 0.3 mg as Na2SeO3
  3. cHemicell™ HT-D, Elanco Animal Health, Guelph, ON, Canada; endo-1,4-β-mannanase (160 × 106 units/kg) from Paenibacillus alvei
  4. dAcid hydrolyzed ether extract
  5. eβ-mannan concentration was calculated using values reported in Shastak et al. [70]
  6. fendo-1,4-β-mannanase activity. 1 IU = the amount of enzyme which generates 0.72 micrograms of reducing sugars per minute from a mannose-containing substrate at pH 7.0 and temperature of 40 °C
  7. gThe lowest detectable limit was 15,000 IU/kg