Fig. 6

Regulation of SREBP1, FASN, PPARG, GPAM, ACACA, AGPAT6, SCD1, PGC-1A and ACSL transcript levels in GMECs transfected with miR-25 mimics or mimic control (miR-NC). Cells were collected 48 h after transfection and RNA was isolated and analyzed for mRNA levels of SREBP1 (a), FASN (b), PPARG (c), GPAM (d), ACACA (e), AGPAT6 (f), SCD1 (g), PGC-1A (h) and ACSL (i) by quantitative real-time PCR. For data normalization, UXT was used as an endogenous control. Transcript expression levels are shown as fold change. The data represent the mean ± S.D. of three independent cell samples. * P < 0.05, ** P < 0.01