Fig. 6

Expression profiling of LrNanog gene. a Relative expression profiling of Nanog in different tissues of L. rohita by quantitative real-time PCR. The data in each tissue was normalized with the value of the reference genes. b Relative expression profiling of Nanog in proliferative spermatogonial stem cells of rohu with comparison to testis. c Western blot showing the presence of Nanog in the testis and in vitro propagated spermatogonial stem cells of rohu with a signal at 42 kDa and intestine (un-related organ) was taken as a control. d Nanog expression during embryonic development from unfertilized eggs to hatching stage in L. rohita. All the data represent the mean of three independent experiments (each in triplicate). Data are shown as mean ± SE