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Table 2 Species-specific primers for the quantification of selected rumen bacterial populations using a real-time qPCR assay

From: Association of residual feed intake with abundance of ruminal bacteria and biopolymer hydrolyzing enzyme activities during the peripartal period and early lactation in Holstein dairy cows

Target bacterial species   Primer sequence (5` → 3`) Reference Efficiencya, %
Anaerovibrio lipolytica F:b
R:c
GAAATGGATTCTAGTGGCAAACG
ACATCGGTCATGCGACCAA
[13] 96.06
Butyrivibrio proteoclasticus F:
R:
GGGCTTGCTTTGGAAACTGTT
CCCACCGATGTTCCTCCTAA
[13] 100.00
Eubacterium ruminantium F:
R:
CTCCCGAGACTGAGGAAGCTTG
GTCCATCTCACACCACCGGA
[53] 106.08
Fibrobacter succinogenes F:
R:
GCGGGTAGCAAACAGGATTAGA
CCCCCGGACACCCAGTAT
[53] 100.67
Megaspheara elsdenii F:
R:
AGATGGGGACAACAGCTGGA
CGAAAGCTCCGAAGAGCCT
[53] 101.35
Prevotella bryantii F:
R:
AGCGCAGGCCGTTTGG
GCTTCCTGTGCACTCAAGTCTGAC
[53] 105.03
Selenomonas ruminantium F:
R:
CAATAAGCATTCCGCCTGGG
TTCACTCAATGTCAAGCCCTGG
[53] 97.91
Succinimonas amylolytica F:
R:
CGTTGGGCGGTCATTTGAAAC
CCTGAGCGTCAGTTACTATCCAGA
[54] 96.80
Streptococcus bovis F:
R:
TTCCTAGAGATAGGAAGTTTCTTCGG
ATGATGGCAACTAACAATAGGGGT
[53] 103.89
Succinivibrio dextrinosolvens F:
R:
TAGGAGCTTGTGCGATAGTATGG
CTCACTATGTCAAGGTCAGGTAAGG
[54] 96.80
Eubacterial primer 1 F:
R:
GGATTAGATACCCTGGTAGT
CACGACACGAGCTGACG
[27] 95.26
Eubacterial primer 2 F:
R:
GTGSTGCAYGGYTGTCGTCA
ACGTCRTCCMCACCTTCCTC
[26] 95.30
Eubacterial primer 3 F: CCTACGGGAGGCAGCAG [29] 99.30
  R: ATTACCGCGGCTGCTGG   
  1. aMeasured efficiencies of the primers in the qPCR reactions
  2. bForward primer
  3. cReverse primer