Association of residual feed intake with abundance of ruminal bacteria and biopolymer hydrolyzing enzyme activities during the peripartal period and early lactation in Holstein dairy cows

Table 2 Species-specific primers for the quantification of selected rumen bacterial populations using a real-time qPCR assay

Target bacterial species		Primer sequence (5` → 3`)	Reference	Efficiency^a, %
Anaerovibrio lipolytica	F:^b R:^c	GAAATGGATTCTAGTGGCAAACG ACATCGGTCATGCGACCAA	[13]	96.06
Butyrivibrio proteoclasticus	F: R:	GGGCTTGCTTTGGAAACTGTT CCCACCGATGTTCCTCCTAA	[13]	100.00
Eubacterium ruminantium	F: R:	CTCCCGAGACTGAGGAAGCTTG GTCCATCTCACACCACCGGA	[53]	106.08
Fibrobacter succinogenes	F: R:	GCGGGTAGCAAACAGGATTAGA CCCCCGGACACCCAGTAT	[53]	100.67
Megaspheara elsdenii	F: R:	AGATGGGGACAACAGCTGGA CGAAAGCTCCGAAGAGCCT	[53]	101.35
Prevotella bryantii	F: R:	AGCGCAGGCCGTTTGG GCTTCCTGTGCACTCAAGTCTGAC	[53]	105.03
Selenomonas ruminantium	F: R:	CAATAAGCATTCCGCCTGGG TTCACTCAATGTCAAGCCCTGG	[53]	97.91
Succinimonas amylolytica	F: R:	CGTTGGGCGGTCATTTGAAAC CCTGAGCGTCAGTTACTATCCAGA	[54]	96.80
Streptococcus bovis	F: R:	TTCCTAGAGATAGGAAGTTTCTTCGG ATGATGGCAACTAACAATAGGGGT	[53]	103.89
Succinivibrio dextrinosolvens	F: R:	TAGGAGCTTGTGCGATAGTATGG CTCACTATGTCAAGGTCAGGTAAGG	[54]	96.80
Eubacterial primer 1	F: R:	GGATTAGATACCCTGGTAGT CACGACACGAGCTGACG	[27]	95.26
Eubacterial primer 2	F: R:	GTGSTGCAYGGYTGTCGTCA ACGTCRTCCMCACCTTCCTC	[26]	95.30
Eubacterial primer 3	F:	CCTACGGGAGGCAGCAG	[29]	99.30
	R:	ATTACCGCGGCTGCTGG

ISSN: 2049-1891