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Fig. 3 | Journal of Animal Science and Biotechnology

Fig. 3

From: Endoplasmic reticulum stress-induced apoptosis in intestinal epithelial cells: a feed-back regulation by mechanistic target of rapamycin complex 1 (mTORC1)

Fig. 3

Effect of tunicamycin on ER stress pathway activation in IEC-6 cells. Cells were incubated with 1 μg/mL tunicamycin for indicated time periods, Western bolt was performed to analyze protein abundance for: (a) ER stress markers, including BiP, IRE-1α, p-IRE1α, eIF2α, p-eIF2α, and ATF4; and (b) apoptosis related proteins, including CHOP, JNK, p-JNK, and Bcl-2. The right panel histograms represent the statistical analysis of protein abundance from three individual experiments. Data are expressed as mean ± SEM. *P < 0.05. TUN, tunicamycin; UT, untreatment

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