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Fig. 1 | Journal of Animal Science and Biotechnology

Fig. 1

From: Endoplasmic reticulum stress-induced apoptosis in intestinal epithelial cells: a feed-back regulation by mechanistic target of rapamycin complex 1 (mTORC1)

Fig. 1

Tunicamycin treatment results in apoptotic cell death in IEC-6 cells. a Cells treated with tunicamycin (0, 1, 5, and 10 μg/mL) for 24 h were harvested for viability assay using a cell counting kit. Data are expressed as mean ± SEM in percentage compared with the control (0 μg/mL of tunicamycin), n = 6. *P < 0.05. b Morphological changes induced by indicated concentrations of tunicamycin (0, 1, 5, and 10 μg/mL) in IEC-6 cells stained with Hoechst 33342. The images were acquired by using a fluorescence microscope. Scale bar: 10 μm. c Flow cytometry analysis of apoptosis in IEC-6 cells by Annexin V-FITC/PI double staining. Cells in the right lower and right upper quadrant were considered as the early and late apoptotic cells, respectively. d The bar chart represents the percentage of apoptotic cells. Data are shown as mean ± SEM, n = 3. *P < 0.05. e Western blot results for the protein levels of cleaved caspase-3 in IEC-6 cells treated with (1 μg/mL) or without tunicamycin for indicated time periods. β-actin was used as a loading control. The histogram represent the statistical analysis of cleaved-caspase-3 of three individual experiments. Data are expressed as mean ± SEM. *P < 0.05. TUN, tunicamycin; UT, untreatment; cleaved-casp-3, cleaved-caspase-3

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