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Table 5 Cell cycle progression of ruminal epithelial cells of hay-fed (CON) and high-grain-fed sheep (G7–28)

From: Morphological adaptation of sheep’s rumen epithelium to high-grain diet entails alteration in the expression of genes involved in cell cycle regulation, cell proliferation and apoptosis

Item1 Treatments SEM Contrast, P
CON G7 G14 G28 Treatment Linear Quadratic Cubic
G0/G1, % 91.77a 89.96a 85.86b 84.73b 0.76 < 0.001 < 0.001 0.754 0.238
S, % 2.78b 2.56b 3.05b 5.11a 0.33 0.007 0.006 0.058 0.691
G2/M, % 4.24b 5.62b 9.70a 9.36a 0.56 < 0.001 < 0.001 0.058 0.004
Apoptosis cells, % 62.72a 19.03d 28.22c 38.33b 3.82 < 0.001 < 0.001 < 0.001 < 0.001
  1. Sheep assigned to CON (n = 5), G7 (n = 5), G14 (n = 5) and G28 (n = 5) received a high-grain diet for 0, 7, 14 and 28 d, respectively
  2. SEM standard error of mean
  3. a-d Means within a row with different superscripts differ (P < 0.05)
  4. 1 G0/G1 = the proportion of cells in the gap G0 and G1 phases (the percentage is based on 10,000 cells); S = the proportion of cells in the synthesis (S) phase, based on the fact that the single-cell DNA level is greater than the DNA content of resting diploid cells (the percentage is based on 10,000 cells measured); G2/M = the proportion of mitotic cells (the percentage is based on 10,000 cells). Apoptosis cells = the proportion of cells in the apoptosis phase (the percentage is based on 10,000 cells measured)