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Table 1 Ingredient and chemical composition of the diet (DM basis)

From: Morphological adaptation of sheep’s rumen epithelium to high-grain diet entails alteration in the expression of genes involved in cell cycle regulation, cell proliferation and apoptosis

Item Diet
Hay High-grain diet
Ingredient composition, % DM
 Oat hay 63.40 26.00
 Alfalfa hay 33.00 14.00
 Corn meal 0 34.20
 Wheat meal 0 18.00
 Soybean meal 0 4.20
 CaCO3 1.00 1.00
 NaCl 0.40 0.40
 CaHPO4 1.20 1.20
 Mineral and vitamin supplementa 1.00 1.00
Nutrient compositionb
 DE, MJ/kg DM 8.88 11.73
 CP, % DM 11.18 11.92
 Crude fat, % DM 2.09 2.49
 Crude fiber, % DM 28.41 12.90
 NDF,% DM 44.45 24.54
 ADF, % DM 19.52 10.15
 Crude ash, % DM 8.34 4.53
 Starch, % DM 3.25 32.34
  1. aContained 16% calcium carbonate; 102 g/kg of Zn; 47 g/kg of Mn; 26 g/kg of Cu; 1,140 mg/kg of I; 500 mg/kg of Se; 340 mg/kg of Co; 17,167,380 IU/kg of vitamin A; 858,370 IU/kg of vitamin D; and 23,605 IU/kg of vitamin E
  2. bValues were analyzed based on the Feed Database in China [60]
  3. Sheep assigned to CON (n = 5), G7 (n = 5), G14 (n = 5) and G28 (n = 5) received a high-grain diet for 0, 7, 14 and 28 d, respectively