Fig. 3From: Morphological adaptation of sheep’s rumen epithelium to high-grain diet entails alteration in the expression of genes involved in cell cycle regulation, cell proliferation and apoptosisExpression of proteins related to cell cycle regulators in rumen epithelial tissue of hay-fed (CON) and high-grain-fed sheep (G7–28). Protein extracts of rumen epithelium samples were prepared and immunoblotted with specific antibodies (a). Intensities of Cyclin D1, Cyclin A2, Cyclin E1, CDK-2, CDK-4 and CDK-6 were normalized to corresponding GAPDH levels (b) (means ± SE; n = 5). Sheep assigned to CON(n = 5), G7(n = 5), G14(n = 5) and G28(n = 5) received a high-grain diet for 0, 7, 14 and 28 d, respectively. Contrast patterns: L = linear, Q = quadratic, C = cubicBack to article page