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Fig. 3 | Journal of Animal Science and Biotechnology

Fig. 3

From: Morphological adaptation of sheep’s rumen epithelium to high-grain diet entails alteration in the expression of genes involved in cell cycle regulation, cell proliferation and apoptosis

Fig. 3

Expression of proteins related to cell cycle regulators in rumen epithelial tissue of hay-fed (CON) and high-grain-fed sheep (G7–28). Protein extracts of rumen epithelium samples were prepared and immunoblotted with specific antibodies (a). Intensities of Cyclin D1, Cyclin A2, Cyclin E1, CDK-2, CDK-4 and CDK-6 were normalized to corresponding GAPDH levels (b) (means ± SE; n = 5). Sheep assigned to CON(n = 5), G7(n = 5), G14(n = 5) and G28(n = 5) received a high-grain diet for 0, 7, 14 and 28 d, respectively. Contrast patterns: L = linear, Q = quadratic, C = cubic

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