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Table 2 Primer sequences used for RT-PCR, in situ hybridization, and qRT-PCR

From: Acquisition of pluripotency in the chick embryo occurs during intrauterine embryonic development via a unique transcriptional network

Gene

Accession no.

Forward (5′ → 3′)

Reverse (5′ → 3′)

Amplicon size, bp

RT-PCR or in situ hybridization

 cNANOG

NM_001146142

CAGCAGACCTCTCCTTGACC

AAGCCCTCATCCTCCACAGC

586

 cPOUV

NM_001309372

GCCAAGGACCTCAAGCACAA

ATGTCACTGGGATGGGCAGA

511

 cSOX2

NM_205188

CACAACTCGGAGATCAGCAA

GTAGGTAGGCGATCCGTTCA

471

 cSOX3

NM_204195

CGGCACCGTACCACTAACTC

GACTCGGAAGCGAACAAAAC

302

 cGAPDH

NM_204305

CACAGCCACACAGAAGACGG

CCATCAAGTCCACAACACGG

443

qRT-PCR

 cGAPDH

NM_204305

ACACAGAAGACGGTGGATGG

GGCAGGTCAGGTCAACAACA

193

 cNANOG

NM_001146142

CAGCAGACCTCTCCTTGACC

AAAAGTGGGGCGGTGAGATG

187

 cPOUV

NM_001309372

TGAAGGGAACGCTGGAGAGC

ATGTCACTGGGATGGGCAGAC

231

 cSOX3

NM_204195

CGGCACCGTACCACTAACTC

GACTCGGAAGCGAACAAAAC

302

 cENS-1

NM_001080873

TGCTCGGCCTTCTGTATCAG

TTCCTCTCGGAACTCCACAG

181

 cTFCP2L1

XM_422087

TCAGCACATTAAAAGCTGAAAGCA

AGCAATCTCAGTGAGGCACTA

110

 cTBX3

NM_001270878

GTGGAAGACGACCCGAAAGT

CACCATCTCCGTGCCTCTTT

78

 cPRDM14

XM_015282907

AAATTCCCCTGCCACCTCTG

CCCGCATGTGTTTGTTCAGG

154

 cKIT

NM_204361

AGCGAACTTCACCTTACCCG

CTGGGAATCCAGTTGCCACA

181

 cLIN28A

NM_001031774

CCGAGAATGAGTCCCAACCC

GGTGAATTCAACGGCTTCGC

197

 cMYC

NM_001030952

GAGGAGAACGACAAGAGGCG

CACGCAGGGCAAAGAAACTC

85

 cSALL4

NM_001080872

AATTCTGCCAGACGGGGAAG

GCTATGCCATTGCTGAGCAC

170