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Fig. 4 | Journal of Animal Science and Biotechnology

Fig. 4

From: Acquisition of pluripotency in the chick embryo occurs during intrauterine embryonic development via a unique transcriptional network

Fig. 4

Expression profiling of cSOX2 and cSOX3 during chicken embryonic development. a In situ hybridization was performed on the whole-mount and longitudinal sections in pre-oviposition stages of chick blastoderm (EGK.III, EGK.VIII and EGK.X) to detect cSOX2 transcripts. b cSOX2 transcripts are clearly detected at neural induction stages between HH 6 and 8 by whole-mount in situ hybridization. c RT-PCR was conducted to confirm cSOX2 expression in EGK.X, HH8, PGC, and CEF. d RT-PCR was conducted to examine cSOX3 expression from the oocyte to the EGK.X embryo. CEF was used as a negative control. e The cSOX3 gene expression in oocyte and intrauterine chicken embryos relative to EGK.X was analyzed using qRT-PCR. cGAPDH was used as a reference gene. Results are shown as mean ± standard error of mean (n = 3). Significant differences of the relative gene expression between consecutive developmental stages (Oocyte vs. EGK.I, EGK.I vs. EGK.III, EGK.III vs. EGK.VI, EGK.VI vs. EGK.VIII, and EGK.VIII vs. EGK.X) were represented as *P < 0.05 and *** P < 0.001. Scale bars = 1 mm (whole-mount of embryo) and 200 μm (longitudinal sections of embryo)

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