Proteomic analysis of adipose tissue during the last weeks of gestation in pure and crossbred Large White or Meishan fetuses gestated by sows of either breed

Table 2 Main biological processes and functional categories of proteins in subcutaneous adipose tissue of pig fetuses as affected by genotype

Functional annotation^a	P-value	Enrich.	Identified proteins^b
Cluster 1 (E score = 2.44)			MeiS > LW: SPTAN1, GSN LW ≥ MeiS: ARHGDIA,MYL6, MYL12A, VIM, APOA1, CAPZB F1_LW ≥ F1_MeiS: ARHGDIA, APOA1
Negative regulation of cellular component organization	< 0.001	21.6
Actin capping Regulation of actin filament-based process	< 0.001 0.008	126 20.0
Cluster 2 (E score = 2.17)			MeiS > LW: FABP3, FABP4, TF, CLIC1 LW ≥ MeiS: APOA1 F1_MeiS > F1_LW: FABP3, TF F1_LW ≥ F1_MeiS: APOA1
Lipid binding PPAR signaling pathway	0.02 0.01	133.0 17.0
Cluster 3 (E score = 1.96)			MeiS > LW: HSPA1B, ALB, NME1 LW ≥ MeiS: ARHGDIA, PDIA3, CSTB F1_MeiS > F1_LW: ALDOC, FABP3, TF, NME1 F1_LW ≥ F1_MeiS: ARHGDIA
Regulation of apoptosis Regulation of programmed cell death	0.007 0.007	4.5 4.5
Cluster 4 (E score = 1.74)			LW ≥ MeiS: PRDX6, PDIA6, PDIA3
Cell redox homeostasis	0.004	29.3	LW ≥ MeiS: PRDX6, PDIA6, PDIA3
Cluster 5 (E score = 1.40)			MeiS > LW: TF, ALB LW ≥ MeiS: APOA1 F1_MeiS > F1_LW: TF F1_LW ≥ F1_MeiS: APOA1
Plasma	0.004	25.8
Cluster 6 (E score = 1.23)			MeiS > LW: ALDOC, NME1, FABP3, FABP4, CLIC1, TF, ALB, HSPA1B, GSN F1_MeiS > F1_LW: ALDOC, FABP3, TF, NME1
Response to nutrient levels Response to extracellular stimulus	0.04 0.04	9.4 8.4

^aThe E score of the cluster was measured by minus log transformation of the geometric mean of the modified Fisher Exact EASE Scores of all annotation terms that belong to this cluster, and it was intended to order the relative importance of the different clusters. The fold enrichments (enrich.) of specific individual annotation terms were also indicated for the most biologically-informative terms within each cluster
^bLists of regulated proteins participating to each cluster were indicated. MeiS > LW: proteins having a higher abundance in pure Meishan than in pure Large White genotypes. LW > MeiS: proteins having a higher abundance in pure Large White than in pure Meishan genotypes. F1_MeiS > F1_LW: proteins having a higher abundance in crossbred (F1) fetuses gestated by MeiS sows when compared with those gestated by Large White sows. F1_LW > F1_MeiS: proteins having a higher abundance in crossbred (F1) fetuses gestated by Large White sows when compared with those gestated by Meishan sows

ISSN: 2049-1891