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Table 1 Main biological processes and functional categories of proteins in subcutaneous adipose tissue of pig fetuses as affected by age of development

From: Proteomic analysis of adipose tissue during the last weeks of gestation in pure and crossbred Large White or Meishan fetuses gestated by sows of either breed

Functional annotationa P-value Enrich. Identified proteinsb
Cluster 1 (E score = 3.70)    d 110 ≥ d 90:
 Pyruvate metabolism < 0.001 20.5 LDHB, MDH1, PDHB, AKR1B1,
 Oxidation reduction < 0.001 6.2 ACADS, PTGR2, ALDH9A1, PRDX1, PRDX6
Cluster 2 (E score = 2.55)    d 110 ≥ d 90:
 Glycolysis < 0.001 13.7 LDHB, MDH1, PDHB, ALDOC,
 Hexose process < 0.001 9.0 GALM, GALK1, ALDH9A1
 Generation of precursor metabolites and energy 0.02 4.6 d 90 ≥ d 110: ATP5B
Cluster 3 (E score = 2.46)    d 110 ≥ d 90:
 Actin binding 0.003 7.8 GSN d 90 ≥ d 110: CFL1, VCL, FSCN1, TPM3
Cluster 4 (E score = 2.11)    d 110 ≥ d 90: FABP3, FABP4, APOA1 d 90 ≥ d 110: VCL, APOA1
 Lipid binding < 0.001 85.4
Cluster 5 (E score = 2.01)    d 110 ≥ d 90: HSPD1, HSPA1B d 90 ≥ d 110: HSPA8, CLIC1
 Response to protein stimulus 0.005 10.8
 Molecular chaperone < 0.001 88.8
Cluster 6 (E score = 1.67)    d 110 ≥ d 90: GSN, MYL12A, MYL12B, CRKL d 90 ≥ d 110: CFL1, ACTB, VCL
 Regulation of actin cytoskeleton 0.001 5.3
Cluster 7 (E score = 1.59)    d 110 ≥ d 90: HSPD1, HSPA1B, PHB, PRDX1, ANXA4 d 90 ≥ d 110: CFL1, LGALS1, ARHGDIA
 Regulation of apoptosis 0.020 2.9
Cluster 8 (E score = 1.47)    d 110 ≥ d 90: GSN
 Cytoskeleton organization 0.015 4.0
 Actin filament organization 0.02 12.0 d 90 ≥ d 110: TUBA1B, TUBB, CFL1, FSCN1, TPM3, DES, STMN1
Cluster 9 (E score = 1.46)    d 110 ≥ d 90: CRKL, APOA1 d 90 ≥ d 110: ARHGDIA, CFL1, APOA1
 Ras protein signal transduction 0.005 10.9
 Rho protein signal transduction 0.007 22.8
Cluster 10 (E score = 1.45)    d 110 ≥ d 90: ALDOC, ACADS, FABP3, FABP4 d 90 ≥ d 110: AHSG, CFL1, SERPINA1
 Response to endogenous stimulus 0.002 5.0
 Response to hormone stimulus 0.03 3.9
Cluster 11 (E score = 1.41)    d 110 ≥ d 90: PRDX1, PRDX6 d 90 ≥ d 110: PDIA6
 Cell redox homeostasis 0.020 13.7
Cluster 12 (E score = 1.18)    d 110 ≥ d 90: TPM3, APOA1, VIM d 90 ≥ d 110: ATP5B, CFL1, ACTB, VCL, ARHGDIA, APOA1, VIM
 Cell motion 0.001 4.8
Cluster 13 (E score = 1.10)    d 110 ≥ d 90: CLEC3B, APOA1 d 90 ≥ d 110: AFP, AHSG, SERPINA1, APOA1
 Plasma < 0.001 20.6
  1. aThe E score of the cluster was measured by minus log transformation of the geometric mean of the modified Fisher Exact EASE Scores of all annotation terms that belong to this cluster, and it was intended to order the relative importance of the different clusters. The fold enrichments (enrich.) of specific individual annotation terms were also indicated for the most biologically-informative terms within each cluster
  2. bLists of regulated proteins participating to each cluster were indicated. d 110 > d 90: proteins having a higher abundance in subcutaneous adipose tissue of fetuses at 110 d of gestation than at 90 d of gestation. d 90 > d 110: proteins having a higher abundance in subcutaneous adipose tissue of fetuses at 90 d of gestation than at 110 d of gestation. APOA1 and VIM were represented by spots with an opposite regulation by age of gestation