Effects of Lactobacillus acidophilus on the growth performance and intestinal health of broilers challenged with Clostridium perfringens

Table 3 The sequence of 16S rRNA quantitative real-time PCR primers used to quantify intestinal bacteria

Target	Primer sequence (5′→3′)^b	Amplicon size, bp	Reference
C. perfringens	F: AAAGATGGCATCATCATTCAAC R: TACCGTCATTATCTTCCCCAAA	279	[40]
Escherichia subgroup^a	F: GTTAATACCTTTGCTCATTGA R: ACCAGGGTATCTAATCCTGT	340	[41]
Lactobacillus subgroup	F: AGCAGTAGGGAATCTTCCA R: CACCGCTACACATGGAG	341	[15]

^aThe targeted Escherichia subgroup contained E. coli, Hafnia alvei and Shigella species
^bF forward, R reverse

ISSN: 2049-1891