Table 2 Quantitative real-time PCR primer sequences
Gene name | Forward primer sequence (5′ to 3′) | Reverse primer sequence (5′ to 3′) | GenBank accession number |
|---|---|---|---|
β-actin | GAGAAATTGTGCGTGACATCA | CCTGAACCTCTCATTGCCA | L08165 |
IL-1β | ACTGGGCATCAAGGGCTA | GGTAGAAGATGAAGCGGGTC | NM_204524 |
IL-8 | ATGAACGGCAAGCTTGGAGCTG | TCCAAGCACACCTCTCTTCCATCC | AJ009800 |
IFN-γ | AGCTGACGGTGGACCTATTATT | GGCTTTGCGCTGGATTC | NM_205149.1 |
TNF-α | GAGCGTTGACTTGGCTGTC | AAGCAACAACCAGCTATGCAC | NM_204267 |
IL-10 | CGGGAGCTGAGGGTGAA | GTGAAGAAGCGGTGACAGC | EF554720.1 |
MUC2 | TTCATGATGCCTGCTCTTGTG | CCTGAGCCTTGGTACATTCTTGT | XM_421035 |
CLDN1 | CATACTCCTGGGTCTGGTTGGT | GACAGCCATCCGCATCTTCT | AY750897.1 |
OCLN | ACGGCAGCACCTACCTCAA | GGGCGAAGAAGCAGATGAG | GI:464,148 |
ZO-1 | CTTCAGGTGTTTCTCTTCCTCCTC | CTGTGGTTTCATGGCTGGATC | XM_413773 |