Effects of chronic dexamethasone administration on hyperglycemia and insulin release in goats

Niu, Liqiong; Chen, Qu; Hua, Canfeng; Geng, Yali; Cai, Liuping; Tao, Shiyu; Ni, Yingdong; Zhao, Ruqian

doi:10.1186/s40104-018-0242-4

Journal of Animal Science and Biotechnology

Table 1 PCR primers for glucose metabolism genes

From: Effects of chronic dexamethasone administration on hyperglycemia and insulin release in goats

Gene	Sequence 5′→3’	GenBank accession no.	Product length, bp
GLUT1	Forward: AACCGCAACGAGGAGAACC	NM_001314223.1	155
	Reverse: TGCAGCACCACGGCAAT
GLUT2	Forward: GAGGCATATCAGGACTCTAC	XM_005675321.3	156
	Reverse: AGGGCACCAATAGCAC
SGLT1	Forward: CACCCATCGCAGCAGT	XM_018060890.1	199
	Reverse: CGGGCGTCTTGAATGT
Na-K/ATPase	Forward: CCTCGAAATCCATTGCTTATACC	[38]	144
	Reverse: GACCATGTCCGTTCCCAAGT
GLUT4	Forward: GGCGGATGCTATGGGTC	JQ343218.1	122
	Reverse: ACGGGTTTCAGGCACTTT
PCK1	Forward: ACGCGCTTCCCGAATTCTCA	XM_004014441.1	130
	Reverse: TCCCCAACCTCTTTAGTGAC
PCK2	Forward: AACAGCAGGGACTCATCCGAAA	XM_015096868.1	100
	Reverse: ATCACCGTCTTGCTCTCTACTCGT
PC	Forward: TCGCACCATGTATGTCATCCC	NM_177946	187
	Reverse: AGGCTTTTTTAAAGGCAGAGGG
HK	Forward: GCGGCTCTCTGATAAAACTCTGTTA	AM492192	139
	Reverse: TGAGCCATCGGGAATAGACCTTAC
IGF1R	Forward: GGCTCAACCCAGGGAA	XM_018065947.1	164
	Reverse: CACTATCAACAGAACCGCAAT
IR	Forward: GCCCTGGTGTCACTTTCC	XM_018051134.1	182
	Reverse: GCTGCCTTAGGTTCTGGTT
IRS1	Forward: TGCCTGACCAGCAAGACCA	XM_018058864.1	187
	Reverse: CACCTGCATCCAGAACTCCC
PI3K	Forward: CGAGCATTTCTGCTTTGGG	XM_018047551.1	152
	Reverse: GGTCTTGGAGGCATTGTTCTG
AKT	Forward: CTAAGCAGCGGCTTGGTG	NM_001285750.1	165
	Reverse: GGTCAGGTGGCGTAATGGT
Atrogin1	Forward: TAAACTTGTGCGATGCTAC	XM_005688865.3	167
	Reverse:TGTCATGTGCTCGGATT
MuRF1	Forward:GAGCAAGGCAGGTGAAGG	XM_018058864.1	134
	Reverse: TGGCACGGCAAGATGAC
G6PC	Forward:AATGTCATGTTGTGGTTGGGATTCT	EF062861	158
	Reverse: GCATTGTAGATGCTCTGGATGTGG
GAPDH	Forward:GGGTCATCATCTCTGCACCT	HM043737.1	180
	Reverse: GGTCATAAGTCCCTCCACGA
18S	Forward:GTGATGGGGATCGGGGATTG	[39]	172
	Reverse: GTAGCGACGGGCGGTGTGTA

GLUT1 Glucose transporter type 1, GLUT2 Glucose transporter type 2, SGLT1 Sodium glucose transporter type 1, Na-K/ATPase Sodium-potassium ATPase, GLUT4 Glucose transporter type 4, PCK1 Cytosolic form of phosphoenolpyruvate carboxykinase, PCK2 Mitochondrial form of phosphoenolpyruvate carboxykinase, PC Pyruvate carboxylase, HK Hexokinase, IGF1R Insulin-like growth factor 1 receptor, IR Insulin receptor, IRS1 Insulin receptor substrate1, PI3K Phosphoinositide 3-kinase, AKT AKT serine/threonine kinase 1, Atrogin1 F-box protein 32, MuRF1 Muscle RING-Finger protein 1, G6PC Glucose-6-phosphatase, GAPDH Glyceraldehyde-3-phosphate dehydrogenase

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ISSN: 2049-1891

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