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Table 2 Group-specific 16S–targeted primers and optimized conditions for real-time PCR

From: Effects of dietary Bacillus amyloliquefaciens supplementation on growth performance, intestinal morphology, inflammatory response, and microbiota of intra-uterine growth retarded weanling piglets

Name Sequence, 5′ → 3’a Annealing temperature, °C Product size, bp Reference
Total bacteria ACTCCTACGGGAGGCAGCAG 60 200 [33]
ATTACCGCGGCTGCTGG
Lactobacillus AGCAGTAGGGAATCTTCCA 55 341 [34]
CACCGCTACACATGGAG
Escherichia coli CATGCCGCGTGTATGAAGAA 60 96 [35]
CGGGTAACGTCAATGAGCAAA
Bacillus GCAACGAGCGCAACCCTTGA 60 92 [36]
TCATCCCCACCTTCCTCCGGT
Bifidobacterium CTCCTGGAAACGGGTGG 55 550 [37]
GGTGTTCTTCCCGATATCTACA
  1. aShown as forward primer followed by reverse primer