Effects of dietary Bacillus amyloliquefaciens supplementation on growth performance, intestinal morphology, inflammatory response, and microbiota of intra-uterine growth retarded weanling piglets

Table 2 Group-specific 16S–targeted primers and optimized conditions for real-time PCR

Name	Sequence, 5′ → 3’^a	Annealing temperature, °C	Product size, bp	Reference
Total bacteria	ACTCCTACGGGAGGCAGCAG	60	200	[33]
Total bacteria	ATTACCGCGGCTGCTGG	60	200	[33]
Lactobacillus	AGCAGTAGGGAATCTTCCA	55	341	[34]
Lactobacillus	CACCGCTACACATGGAG	55	341	[34]
Escherichia coli	CATGCCGCGTGTATGAAGAA	60	96	[35]
Escherichia coli	CGGGTAACGTCAATGAGCAAA	60	96	[35]
Bacillus	GCAACGAGCGCAACCCTTGA	60	92	[36]
Bacillus	TCATCCCCACCTTCCTCCGGT	60	92	[36]
Bifidobacterium	CTCCTGGAAACGGGTGG	55	550	[37]
Bifidobacterium	GGTGTTCTTCCCGATATCTACA	55	550	[37]

ISSN: 2049-1891