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Table 3 PCR primers for real-time PCR assay

From: Effects of fumaric acid supplementation on methane production and rumen fermentation in goats fed diets varying in forage and concentrate particle size

Target specie

Primer

Primer sequence (5′ to 3′)

Bacteria a

Forward

CGGCAACGAGCGCAACCC

Reverse

CCATTGTAGCACGTGTGTAGCC

Methanogen b

Forward

GAGGAAGGAGTGGACGACGGTA

Reverse

ACGGGCGGTGTGTGCAAG

B. fibrisolvens b

Forward

GAGGAAGTAAAAGTCGTAACAAGGTTTC

Reverse

CAAATTCACAAAGGGTAGGATGATT

F. succinogenes a

Forward

GTTCGGAATTACTGGGCGTAAA

Reverse

CGCCTGCCCCTGAACTATC

R.flavefaciens a

Forward

CGAACGGAGATAATTTGAGTTTACTTAGG

Reverse

CGGTCTCTGTATGTTATGAGGTATTACC

S. ruminantium b

Forward

GGCGGGAAGGCAAGTCAGTC

Reverse

CCTCTCCTGCACTCAAGAAAGACAG

  1. aDenman and McSweeney, [27]; bKhafipour et al. [28]