Table 3 Relative abundance of microbial species in mixed ruminal fluid from Holstein cows fed a control diet (CON) or CON supplemented with rumen-protected methionine (SM) during the periparturient period
|  |  |  |  |  | Day × Treatment |  |  |  | |||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Â | Treatment | Day | CON | SM | P value1 | ||||||
Species | CON | SM | −10 | 20 | −10 | 20 | −10 | 20 | Trt | Day | T × D |
A. lipolytica | 3.28×10−3 | 3.42×10−3 | 2.50×10-3b | 4.48×10-3a | 2.25×10−3 | 4.77×10−3 | 2.77×10−3 | 4.21×10−3 | 0.82 | <0.01 | 0.42 |
B. proteoclasticus | 1.07×10−2 | 8.88×10−3 | 1.01×10−2 | 9.46×10−3 | 1.24×10−2 | 9.29×10−3 | 8.18×10−3 | 9.64×10−3 | 0.20 | 0.67 | 0.13 |
E. ruminantium | 2.44×10−2 | 2.45×10−2 | 2.23×10−2 | 2.69×10−2 | 2.59×10−2 | 2.31×10−2 | 1.92×10−2 | 3.13×10−2 | 0.98 | 0.38 | 0.16 |
F. succinogenes | 7.10×10−4 | 6.93×10−4 | 1.16×10-3a | 4.25×10-4b | 6.29×10-4B | 8.02×10-4B | 2.13×10-3A | 2.25×10-4C | 0.92 | <0.01 | <0.01 |
M. elsdenii | 3.90×10−5 | 1.90×10−5 | 2.40×10-6b | 2.34×10-4a | 4.60×10−6 | 2.07×10−4 | 1.00×10−6 | 2.65×10−4 | 0.43 | <0.01 | 0.24 |
P. bryantii | 4.22×10−3 | 1.30×10−3 | 7.61×10-4b | 7.20×10-3a | 1.12×10−3 | 1.60×10−2 | 5.19×10−4 | 3.25×10−3 | 0.12 | <0.01 | 0.57 |
S. ruminantium | 4.10×10-1a | 2.98×10-1b | 2.63×10-1b | 4.65×10-1a | 2.63×10-1C | 6.39×10-1A | 2.63×10-1C | 3.37×10-1BC | 0.01 | <0.01 | 0.01 |
S. amylolytica | 1.02×10−4 | 2.30×10−4 | 2.51×10−4 | 9.40×10−5 | 9.90×10-5B | 1.05×10-4B | 6.32×10-4A | 8.40×10-5B | 0.16 | 0.07 | 0.05 |
S. dextrinosolvens | 9.10×10−5 | 1.60×10−4 | 1.71×10-4a | 8.50×10-5b | 8.10×10-5B | 1.02×10-4B | 3.64×10-4A | 7.00×10-5B | 0.10 | 0.05 | 0.01 |
S. bovis | 8.09×10−3 | 3.50×10−3 | 3.17×10-3b | 8.93×10-3a | 6.68×10−3 | 9.79×10−3 | 1.51×10−3 | 8.14×10−3 | 0.22 | 0.02 | 0.14 |