Fig. 2
From: Use of gene-editing technology to introduce targeted modifications in pigs
Genotyping results from single blastocysts injected with CRISPR/Cas9 system. All PCR products were digested with NheI. PCR product size from wild-type genomic DNA was 1.1Â kb. If the embryo carried modified allele through HDR, we expected to see two fragments (950Â bp and 160Â bp) after digestion with NheI. Genomic DNA from an embryo carrying homozygous HDR mutation was served as a positive control (P). * indicates embryos carrying knock-in events. L is a molecular ladder