Fig. 1
From: Use of gene-editing technology to introduce targeted modifications in pigs
Strategy of inducing HDR during embryogenesis to disrupt RAG2. Two black bars indicate target sites by CRISPR/Cas9 system on RAG2 exon. Red bar on the donor DNA shows the location of sequences introduced through HDR; yellow sequences are stop codons and green sequences are restriction enzyme sites (NheI and SmaI). Red arrows indicate the location of primers used to amplify the region for genotyping. Blue arrow was used as a primer for Sanger sequencing