Skip to main content
Fig. 1 | Journal of Animal Science and Biotechnology

Fig. 1

From: Functional roles of ornithine decarboxylase and arginine decarboxylase during the peri-implantation period of pregnancy in sheep

Fig. 1

Evidence for delivery of MAOs and knockdown of translation of mRNAs for ODC1, ADC and their combination in ovine conceptus trophectoderm on d 16 of pregnancy. [a] These images confirmed uptake of lissamine-tagged MAOs (red staining) in MAO control, MAO-ODC1, MAO-ADC, MAO-ODC1: MAO-ADC (a) and MAO-ODC1: MAO-ADC (b). [b] The results indicate that translation of ODC1 mRNA was not inhibited in MAO-control conceptuses (Panel B1) as noted by abundant green staining; however, green staining for ODC1 is very weak for the conceptuses exposed to MAO-ODC1, and MAO-ODC1:MAO-ADC. [c] Immunofluorescence images indicate abundant green staining for ADC in the MAO-contro conceptuses, but not in the MAO-ADC or MAO-ODC1:MAO-ADC conceptuses. These results confirmed that the respective MAOs inhibited translation of ODC1 and ADC as expected. Purified non-relevant rabbit IgG was substituted for the primary antibody as the negative control as evidenced by the lack of green staining

Back to article page

Journal of Animal Science and Biotechnology

ISSN: 2049-1891