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Table 2 The effect of seeding density on the growth of testis cells in culture

From: Optimization of culture conditions for short-term maintenance, proliferation, and colony formation of porcine gonocytes

Cell seeding density

Maximum confluency

Confluency at days

Cell count

Viability, %

1.0 × 105

(*~30% at 7)

(*2.98 × 105 ± 0.01)

(*88.4 ± 1.02)

3.0 × 105

>90% at 6.0 ± 0.17 a

8.10 × 105 ± 0.01

91.21 ± 0.58

5.0 × 106

>90% at 4.0 ± 0.15 b

8.16 × 105 ± 0.01

91.62 ± 0.56

  1. The results are mean ± SEM
  2. *The results from 1.0 × 105 seeding density group were excluded from statistical analysis as the cells did not reach 90% confluency
  3. Independent sample t test was used for statistical analyses
  4. abWithin each column, data with different superscripts differ significantly (P < 0.05). n = 12 replications
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Journal of Animal Science and Biotechnology

ISSN: 2049-1891