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Table 1 The population of testis cells obtained after enzymatic digestion and subsequent enrichment

From: Optimization of culture conditions for short-term maintenance, proliferation, and colony formation of porcine gonocytes

Method

Proportion, %

Cell count, × 106 *

Cell loss, % **

Viability, %

In vitro growth

Gonocytes

Somatic cells

Total

Gonocytes

Total

Gonocytes

Enzymatic digestion

40.60 ± 1.21 a

59.40 ± 1.21 a

37.84 ± 2.44 a

15.38 ± 1.09 a

92.62 ± 0.59 a

Yes

Nycodenz centrifugation

80.40 ± 1.46 b

19.6 0 ± 1.46 b

10.00 ± 0.41b

8.00 ± 0.21 b

73.18 ± 1.76 a

46.72 ± 4.69 a

84.20 ± 1.06 b

Limited

ECM differential plating

86.40 ± 1.03 c

13.6 0 ± 1.03 c

3.02 ± 0.19 c

2.60 ± 0.2 c

91.62 ± 0.79 b

82.61 ± 1.71 b

81.66 ± 0.71 c

Limited

  1. The results are mean ± SEM
  2. *Each replicate was based on the digestion of ~600 mg of testis tissue
  3. One-way ANOVA (or t test **) was used for statistical analyses, and P < 0.05 was considered as significant
  4. abcWithin each column, data with different superscripts differ significantly (P < 0.05). n = 5 replications