Method | Proportion, % | Cell count, × 106 * | Cell loss, % ** | Viability, % | In vitro growth | |||
---|---|---|---|---|---|---|---|---|
Gonocytes | Somatic cells | Total | Gonocytes | Total | Gonocytes | |||
Enzymatic digestion | 40.60 ± 1.21 a | 59.40 ± 1.21 a | 37.84 ± 2.44 a | 15.38 ± 1.09 a | – | – | 92.62 ± 0.59 a | Yes |
Nycodenz centrifugation | 80.40 ± 1.46 b | 19.6 0 ± 1.46 b | 10.00 ± 0.41b | 8.00 ± 0.21 b | 73.18 ± 1.76 a | 46.72 ± 4.69 a | 84.20 ± 1.06 b | Limited |
ECM differential plating | 86.40 ± 1.03 c | 13.6 0 ± 1.03 c | 3.02 ± 0.19 c | 2.60 ± 0.2 c | 91.62 ± 0.79 b | 82.61 ± 1.71 b | 81.66 ± 0.71 c | Limited |