Fig. 5

Number of gonocytes or gonocyte colonies observed after applying different medium changing regimens. The cultured cells underwent one of the following medium changing regimens: 1) Seeded with 3 mL of medium and the medium was routinely changed every 2 d (Routine); 2) Seeded with 2 mL of medium, followed by the addition of another 1 mL after 24 h, and then every 2 d, 1 mL of medium was removed from the well and replaced with 1 mL of fresh medium (Modified); 3) Seeded with 3 mL of medium but the medium was not changed for 7 d (Unchanged). The data are mean ± SEM. ^ab Data with different superscripts within each day differ significantly (P < 0.05). ^xy Data with different superscripts within each treatment over time differ significantly (P < 0.05). n = 5 replications