Fig. 4
Number of gonocytes upon analysis of the floating cells from the removed medium. The initial number of cells at seeding was set at 3.0 × 105 cells/well containing ~40% gonocytes and the viability was 89 ± 0.5%. The medium removed from the plates was examined and large numbers of gonocytes were seen floating in the medium on Day 2. Most of the floating gonocytes were eliminated by routine (every 2 d) medium changes (Routine), while some were present on Days 4 and 6, if the medium was not changed (Unchanged). The data are mean ± SEM. ab Data with different superscripts within each day differ significantly (P < 0.05). xyz Data with different superscripts within each treatment over time differ significantly (P < 0.05). n = 5 replications