Effects of Bacillus coagulans supplementation on the growth performance and gut health of broiler chickens with Clostridium perfringens-induced necrotic enteritis

Table 8 Effect of Bacillus coagulans on immune-related gene expressions in the jejunum of broilers challenged with NE (n = 6)

Items	NE^c	MUC2		TLR2		TLR4		IFN-γ		TNFSF15		LYZ		Fowlicidin-2
Items	NE^c	7 DPI^d	14 DPI	7 DPI	14 DPI	7 DPI	14 DPI	7 DPI	14 DPI	7 DPI	14 DPI.	7 DPI	14 DPI	7 DPI	14 DPI
Bacillus coagulans, mg/kg
0	–	1.17	0.79	1.01	1.07	1.04	1.19	1.06	1.01	1.01	1.18	1.16	1.10	1.07	1.53
0	+	1.02	0.36	0.48	0.30	0.62	0.43	2.36	1.22	0.58	0.25	0.76	0.25	0.85	0.54
400	–	1.47	0.94	0.90	1.05	0.99	1.12	0.95	0.86	1.09	1.35	1.65	0.84	1.70	1.79
400	+	1.09	0.49	0.50	0.46	0.39	0.62	2.58	0.74	0.79	0.39	1.71	0.46	0.64	1.13
SEM^e		0.101	0.077	0.058	0.054	0.053	0.120	0.206	0.089	0.074	0.070	0.133	0.072	0.107	0.188
Main effect
400		1.28	0.71	0.70	0.76	0.69	0.87	1.77	0.80	0.94	0.87	1.68^a	0.65	1.17	1.46
0		1.09	0.57	0.74	0.69	0.83	0.81	1.71	1.12	0.79	0.72	0.96^b	0.67	0.96	1.04
Challenged		1.05	0.42^b	0.49^b	0.38^b	0.50^b	0.52^b	2.47^a	0.98	0.68^b	0.32^b	1.23	0.36^b	0.74^b	0.83^b
Non-challenged		1.32	0.86^a	0.95^a	1.06^a	1.01^a	1.15^a	1.01^b	0.94	1.05^a	1.27^a	1.40	0.97^a	1.39^a	1.66^a
P-values^f
Bacillus coagulans		0.370	0.367	0.714	0.531	0.191	0.804	0.892	0.089	0.335	0.289	0.011	0.870	0.335	0.268
Challenged		0.204	0.008	<0.001	<0.001	<0.001	0.014	0.001	0.804	0.020	<0.001	0.527	<0.001	0.006	0.036
Challenged × Bacillus coagulans		0.562	0.935	0.594	0.397	0.393	0.603	0.679	0.364	0.670	0.924	0.393	0.114	0.057	0.671

^a,bMeans within the same column with different superscripts differ significantly (P < 0.05)
^cNE = Co-challenged with Eimeria spp. and Clostridium perfringens; −, without NE challenge; +, with NE challenge
^dDPI = days post infection
^eSEM, standard error of the mean
^fP-values represent the main effect of the diet, the main effect of NE challenge, and the interaction between the dietary treatments and NE challenge

ISSN: 2049-1891