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Table 5 Effect of Bacillus coagulans on jejunal morphology and goblet cell numbers of broiler chickens challenged with NE (n = 6)

From: Effects of Bacillus coagulans supplementation on the growth performance and gut health of broiler chickens with Clostridium perfringens-induced necrotic enteritis

  Villous height, μm Crypt depth, μm VH/CDf GC cellsg
Items NEe d 28 d 42 d 28 d 42 d 28 d 42 d 28 d 42
Bacillus coagulans, mg/kg
 0 1248 1161 281 218 4.65 5.45 44.3 45.5
 0 + 1048 1139 294 239 3.65 4.89 42.3 42.0
 400 1289 1527 241 202 5.99 7.93 52.5 53.3
 400 + 1208 1290 233 209 5.41 6.55 53.0 47.0
 SEMh   34.5 29.8 8.9 7.2 0.292 0.208 1.37 0.68
Main effect
 400   1249 1409a 237b 206 5.70a 7.24a 52.8a 50.1a
 0   1148 1150b 288a 228 4.15b 5.17b 43.3b 43.8b
 Challenged   1128 1215 264 224 4.53 5.72d 47.6 44.5d
 Non-challenged   1269 1344 261 211 5.32 6.69c 48.4 49.4c
P-valuesi
Bacillus coagulans   0.169 <0.001 0.015 0.141 0.019 <0.001 0.005 0.001
 Challenged   0.065 0.051 0.877 0.363 0.196 0.028 0.788 0.004
 Challenged ×Bacillus coagulans   0.408 0.096 0.551 0.645 0.724 0.306 0.656 0.329
  1. a, b, c, d Means within the same column with different superscripts differ significantly (P < 0.05)
  2. eNE = Co-challenged with Eimeria spp. and Clostridium perfringens; −, without NE challenge; +, with NE challenge
  3. fVH/CD = villus height to crypt depth ratio
  4. gGC cells = goblet cells numbers per mm2
  5. hSEM, standard error of the mean
  6. iP-values represent the main effect of the diet, the main effect of NE challenge, and the interaction between the dietary treatments and NE challenge