Effects of Bacillus coagulans supplementation on the growth performance and gut health of broiler chickens with Clostridium perfringens-induced necrotic enteritis

Table 2 Sequences of the oligonucleotide primers used for quantitative real-time PCR^a

Gene	Primer sequence 5′ → 3′	GenBank Accession No.	Efficiency^b, %
GAPDH	F: GGTGGTGCTAAGCGTGTTAT	K01485	93.23
GAPDH	R: ACCTCTGTCATCTCTCCACA	K01485	93.23
TNFSF15	F: CCTGAGTATTCCAGCAACGCA	NM010245578	113.59
TNFSF15	R: ATCCACCAGCTTGATGTCACTAAC	NM010245578	113.59
IFN-γ	F: AGCTGACGGTGGACCTATTATT	Y07922	95.29
IFN-γ	R: GGCTTTGCGCTGGATTC	Y07922	95.29
TLR-2	F: CTGGGAAGTGGATTGTGGA	AB050005.2	93.39
TLR-2	R: AAGGCGAAAGTGCGAGAAA	AB050005.2	93.39
TLR-4	F: GGATCTTTCAAGGTGCCACA	AY064697	98.08
TLR-4	R: CAAGTGTCCGATGGGTAGGT	AY064697	98.08
MUC-2	F: GGGATATTCACCTGGAGAAACCATT	XM_421045	100.41
MUC-2	R: TTGCATGTCCATCTGCCTGAA	XM_421045	100.41
LYZ	F: CCCAGGCTCCAGGAACCT	NM_205281	98.42
LYZ	R: CACGCTCGCTGTTATGTCTGA	NM_205281	98.42
Fowlicidin-2	F: CAAGGAGAATGGGGTCATCAG	NM_001605.3	85.54
Fowlicidin-2	R: CGTGGCCCCATTTATTCATTCA	NM_001605.3	85.54

F forward, R reverse, GAPDH, glyceraldehyde-3-phosphate dehydrogenase, IFN-γ interferon-γ, IL interleukin, LYZ lysozyme, TLR toll-like receptor, TNFSF15 tumor necrosis factor super family 15
^aPrimers were designed and synthesized by Sango Biotech (Shanghai) Co., Ltd
^bPCR amplification efficiency of primers of each gene

ISSN: 2049-1891