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Fig. 5 | Journal of Animal Science and Biotechnology

Fig. 5

From: Functional characterization of oligopeptide transporter 1 of dairy cows

Fig. 5

Kinetic analysis of Gly-Sar transport by bPepT1 that was measured in bPepT1-transfected Chinese hamster ovary cells. bPepT1-transfected (filled dots) and pcDNA3.1-transfected (empty dots) cells were incubated with five different concentrations of Gly-Sar (0.02–10 mmol/L, with 3.7 × 104 Bq/mL [3H]-Gly-Sar) for 5 min. The cells transfected with the pcDNA3.1 control vector were used as a control. The values are means (n = 6) with their standard errors. Inset: Eadie-Hofstee plot of the Gly-Sar uptake in bPepT1-transfected cells after corrected by the uptake of empty vector-transfected cells at the individual concentrations

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