Target genes | GenBank accession | Primer sequences (5′ to 3′) | PCR products, bp |
---|---|---|---|
Primers used for QRT-PCR: | |||
 BHMT | XM_414685.3 | F: TCTTCCTGAATTTCCCTT R: TGAACATCCCATCTAGTGA | 57 |
 GNMT | XM_015283546.1 | F: GGAGGAGGGCTTCCAAGTGA R: GCTCCAGCGTCAGCCAGTT | 140 |
 DNMT1 | NM_206952.1 | F: CGAGTGGGACGGCTTCTT R: AGGCGATAGGTGTCAGGGA | 144 |
 VTGII | F: GAATCCTTCTCGACAAGCCAG R: GCTTCAGCAAAGACGTTCCAG | 186 | |
 ERα | NM_205183.2 | F: TAGTTCCGCTCTACGACCTCTT R: AGTTGGTTTCGGTTCTCCTCTT | 106 |
 ERβ | F: AGCGTGTTATGGTCTGCTCC R: GCTCTTAGGCTGCTCTGCAT | 87 | |
 GR | NM_001037826.1 | F: CTTCCATCCGCCCTTCA R: TCGCATCTGTTTCACCC | 203 |
 β-actin | NM_205518.1 | F: ATG GCTCCGGTATGTGCA A R: TGTCTTTCTGGCCCATACCAA | 120 |
Primers used for Bisulfite sequencing of GR promoter: | Â | ||
 Segment 1 |  | F: TGTTATTTTGTAGAAGTGGGTGTGTTAG R: TAAACAACTTCRCACAACCCATTC |  |
 Segment 2 |  | F: GAATGGGTTGTGYGAAGTTGTTTA R: TTAAAATAACCCRACCRAATACTCC |  |
 Segment 3 |  | F: GTAYGGAGTYGTTTTGTTGGTTG R: ACTATCCTCCAAACCCATCTAATACC |  |
 Segment 4 |  | F: TTATTTGTAAATGTGGGGTTTATTTATAG R: TATAAACACTCCCCAACTCTACTATAACC |  |