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Table 2 Nucleotide sequences of specific primers

From: Dietary betaine activates hepatic VTGII expression in laying hens associated with hypomethylation of GR gene promoter and enhanced GR expression

Target genes

GenBank accession

Primer sequences (5′ to 3′)

PCR products, bp

Primers used for QRT-PCR:

 BHMT

XM_414685.3

F: TCTTCCTGAATTTCCCTT

R: TGAACATCCCATCTAGTGA

57

 GNMT

XM_015283546.1

F: GGAGGAGGGCTTCCAAGTGA

R: GCTCCAGCGTCAGCCAGTT

140

 DNMT1

NM_206952.1

F: CGAGTGGGACGGCTTCTT

R: AGGCGATAGGTGTCAGGGA

144

 VTGII

NM_001031276.1

F: GAATCCTTCTCGACAAGCCAG

R: GCTTCAGCAAAGACGTTCCAG

186

 ERα

NM_205183.2

F: TAGTTCCGCTCTACGACCTCTT

R: AGTTGGTTTCGGTTCTCCTCTT

106

 ERβ

NM_204794.2

F: AGCGTGTTATGGTCTGCTCC

R: GCTCTTAGGCTGCTCTGCAT

87

 GR

NM_001037826.1

F: CTTCCATCCGCCCTTCA

R: TCGCATCTGTTTCACCC

203

 β-actin

NM_205518.1

F: ATG GCTCCGGTATGTGCA A

R: TGTCTTTCTGGCCCATACCAA

120

Primers used for Bisulfite sequencing of GR promoter:

 

 Segment 1

 

F: TGTTATTTTGTAGAAGTGGGTGTGTTAG

R: TAAACAACTTCRCACAACCCATTC

 

 Segment 2

 

F: GAATGGGTTGTGYGAAGTTGTTTA

R: TTAAAATAACCCRACCRAATACTCC

 

 Segment 3

 

F: GTAYGGAGTYGTTTTGTTGGTTG

R: ACTATCCTCCAAACCCATCTAATACC

 

 Segment 4

 

F: TTATTTGTAAATGTGGGGTTTATTTATAG

R: TATAAACACTCCCCAACTCTACTATAACC

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