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Table 2 Nucleotide sequences of specific primers

From: Dietary betaine activates hepatic VTGII expression in laying hens associated with hypomethylation of GR gene promoter and enhanced GR expression

Target genes GenBank accession Primer sequences (5′ to 3′) PCR products, bp
Primers used for QRT-PCR:
BHMT XM_414685.3 F: TCTTCCTGAATTTCCCTT
R: TGAACATCCCATCTAGTGA
57
GNMT XM_015283546.1 F: GGAGGAGGGCTTCCAAGTGA
R: GCTCCAGCGTCAGCCAGTT
140
DNMT1 NM_206952.1 F: CGAGTGGGACGGCTTCTT
R: AGGCGATAGGTGTCAGGGA
144
VTGII NM_001031276.1 F: GAATCCTTCTCGACAAGCCAG
R: GCTTCAGCAAAGACGTTCCAG
186
ERα NM_205183.2 F: TAGTTCCGCTCTACGACCTCTT
R: AGTTGGTTTCGGTTCTCCTCTT
106
ERβ NM_204794.2 F: AGCGTGTTATGGTCTGCTCC
R: GCTCTTAGGCTGCTCTGCAT
87
GR NM_001037826.1 F: CTTCCATCCGCCCTTCA
R: TCGCATCTGTTTCACCC
203
β-actin NM_205518.1 F: ATG GCTCCGGTATGTGCA A
R: TGTCTTTCTGGCCCATACCAA
120
Primers used for Bisulfite sequencing of GR promoter:  
 Segment 1   F: TGTTATTTTGTAGAAGTGGGTGTGTTAG
R: TAAACAACTTCRCACAACCCATTC
 
 Segment 2   F: GAATGGGTTGTGYGAAGTTGTTTA
R: TTAAAATAACCCRACCRAATACTCC
 
 Segment 3   F: GTAYGGAGTYGTTTTGTTGGTTG
R: ACTATCCTCCAAACCCATCTAATACC
 
 Segment 4   F: TTATTTGTAAATGTGGGGTTTATTTATAG
R: TATAAACACTCCCCAACTCTACTATAACC