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Table 1 Composition of the experimental diets

From: Dietary betaine activates hepatic VTGII expression in laying hens associated with hypomethylation of GR gene promoter and enhanced GR expression

Ingredient, % Control Betaine
Corn 65.00 65.00
Soybean meal 24.67 24.67
Shell powder 6.70 6.70
Limestone 2.03 2.03
Salt 0.30 0.30
Dicalcium phosphate 0.83 0.83
Zeolite 0.01 0.01
Choline chloride 0.17 0.17
Methionine 0.12 0.12
Vitamin premixa 0.03 0.03
Minerals premixb 0.10 0.10
Betaine 0 0.5
  1. aThe vitamins premix contain (per kg): vitamin D3: 9,000,000 IU; vitamin K: 35,000,000 IU; vitamin B1: 10 g; vitamin B2: 28 g; vitamin B6: 12 g; vitamin B12: 80 mg; vitamin E: 140 g; vitamin K3: 9 g; D-biotin: 5.60 g; D-pantothenic acid: 36 g; folic acid: 3.5 g; niacinamide: 100 g; ethoxyquin:1.65 g
  2. bThe minerals premix contain (per kg): Cu: 6.4 g; Fe: 72 g; Zn: 64 g; Mn: 72 g; Se: 240 mg; I: 480 mg