Table 1 Gene name, accession number, forward and reverse primer sequences used for qPCR analysis
Gene Name | Gene ID | Sequence ID | Forward primer sequence (5′–3′) | Reverse sequence (5′–3′) | Primer efficiency, % | Amplicon length, bp |
|---|---|---|---|---|---|---|
Progesterone receptor | PGR | NM_001205356.1 | GCCGCAGGTCTACCAGCCCTA | GTTATGCTGTCCTTCCATTGCCCTT | 96.9 | 199 |
Estrogen receptor 1 | ESR1 | NM_001001443.1 | CAGGCACATGAGCAACAAAG | TCCAGCAGCAGGTCGTAGAG | 99.1 | 82 |
Estrogen receptor 2 | ESR2 | NM_174051.3 | TCACGTCAGGCACGCCAGTAAC | CACCAGGTTGCGCTCAGACCC | 99.5 | 155 |
Patched 2a | PTCH2 | XM_005197904.1 | CATCCTGCTGCTGTGTACTT | ATCGCCAGGACCAGTACTAT | 99.9 | 87 |
Epidermal growth factor receptor | EGFR | XM_002696890.3 | ATGCTCTATGACCCTACCAC | TTCCGTTACAAACTTTGCCA | 97.6 | 178 |
Heparin-binding EGF-like growth factor | HB-EGF | NM_001144090.1 | CATCCACGGAGAATGCAAATAC | CAGCAGACAGACGGATGATAG | 98.6 | 181 |
Collagen, type IV, alpha 1 | COL4A1 | NM_001166511.1 | CACGGCTACTCTTTGCTCTAC | GAAGGGCATGGTACTGAACTT | 96.48 | 102 |
Integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61) | ITGB3 | NM_001206490.1 | GGGAGAGTGCTATGGTTAGA | CTTCACAAGACACCCAAGAG | 92.09 | 142 |
Actin Beta | ACTB | NM_173979.3 | GGATGAGGCTCAGAGCAAGAGA | TCGTCCCAGTTGGTGACGAT | 93.7 | 77 |
Glyceraldehyde-3-Phosphate Dehydrogenase | GAPDH | NM_001034034.2 | GCCATCAATGACCCCTTCAT | TGCCGTGGGTGGAATCA | 99.99 | 69 |
Ribosomal Protein S18 | RPS18 | AY786141.1 | TGGAGAGTATTGCGCCTTCTC | CACAAGTTCCACCACACTATTGG | 97.9 | 79 |